Protein Info for PS417_16375 in Pseudomonas simiae WCS417

Annotation: 2-hydroxyacid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 17 to 327 (311 residues), 97.8 bits, E=6.2e-32
• PF02826: 2-Hacid_dh_C
  • amino acids 110 to 297 (188 residues), 192.9 bits, E=4.8e-61
• PF03446: NAD_binding_2
  • amino acids 146 to 257 (112 residues), 22.6 bits, E=1.4e-08

Best Hits

Swiss-Prot: 54% identical to DDH_HAEIN: 2-hydroxyacid dehydrogenase homolog (ddh) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03778, D-lactate dehydrogenase [EC: 1.1.1.28] (inferred from 96% identity to pfs:PFLU3778)

MetaCyc: 55% identical to D-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
D-lactate dehydrogenase. [EC: 1.1.1.28]

Predicted SEED Role

"D-lactate dehydrogenase (EC 1.1.1.28)" in subsystem Fermentations: Lactate or Fermentations: Mixed acid (EC 1.1.1.28)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (16/17 steps found)
• heterolactic fermentation (14/18 steps found)
• L-alanine degradation II (to D-lactate) (3/3 steps found)
• mixed acid fermentation (12/16 steps found)
• pyruvate fermentation to (R)-lactate (1/1 steps found)
• vancomycin resistance I (1/3 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (5/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (2/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.28

Sequence Analysis Tools

See A0A1N7UMI8 at UniProt or InterPro

Protein Sequence (329 amino acids)

>PS417_16375 2-hydroxyacid dehydrogenase (Pseudomonas simiae WCS417)
MRVILFSSQTYDRDSFLGEPLPPGLELQFQPARLNLDTVALAEHHEVVCAFINDDLSAPV
LEHLAKGGTRLIALRSAGYNHVDLACAKRLGLTIVRVPAYSPHAVAEHAVALILALNRRL
HRAYNRTRDGDFSLHGLTGFDLVGKTVGVVGTGQIGATFAKIMAGFGCQLLAYDPFPNPQ
VQALGTRYVSLPELLAQAQIISLHCPLTADSKHLINARSLAQMQPGAMLINTGRGGLVDT
PALIEALKDGQLGYLGLDVYEEEAQLFFEDRSDLPLQDDVLARLLTFPNVIITAHQAFLT
REALAAIAGTTLANIAAWADGRAQNLVEG