Protein Info for GFF3169 in Xanthobacter sp. DMC5

Annotation: Modulator of FtsH protease HflK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 transmembrane" amino acids 48 to 71 (24 residues), see Phobius details PF12221: HflK_N" amino acids 1 to 50 (50 residues), 34.1 bits, see alignment 2.1e-12 TIGR01933: HflK protein" amino acids 73 to 342 (270 residues), 278.3 bits, see alignment E=3e-87 PF01145: Band_7" amino acids 76 to 268 (193 residues), 98.6 bits, see alignment E=4.4e-32

Best Hits

KEGG orthology group: K04088, membrane protease subunit HflK [EC: 3.4.-.-] (inferred from 85% identity to xau:Xaut_1664)

Predicted SEED Role

"HflK protein" in subsystem Hfl operon

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (377 amino acids)

>GFF3169 Modulator of FtsH protease HflK (Xanthobacter sp. DMC5)
MSWKNQSGGPWGNGPRGPWGSGPSSSGPTPPDLEDLIRRSQDRLRSIMPGSFGINGIILV
IALVVAGWFLSGFYRVLPDEQGVVLRFGKFVGITQPGLNYHWPYPIETVLTPKVTFVNRI
DIGMRVSEDTRRNGTRTTDVAEESLMLTGDENIVDVDFSVFWVINNAEEYLFNVQNPEGT
IKAVAESAMREVIGRTNIQPILTGARQNIETGVQELMQQVLNGYKAGVQVTQVQMQKVDP
PSLVIDAFRDVQAARADAERAQNEAQTYANRVLPEARGEASRIENAAQAYRERTVVEARG
QAARFLKVFDEYKKAKEVTRQRMYLETMERVLGSVDKVLVDPSAARQGGGGVVPVLPLTD
TSRRAPAPATQPQGAAR