Protein Info for GFF3166 in Sphingobium sp. HT1-2

Annotation: SSU ribosomal protein S12p (S23e)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 136 TIGR00981: ribosomal protein uS12" amino acids 14 to 136 (123 residues), 219.1 bits, see alignment E=5.7e-70 PF00164: Ribosom_S12_S23" amino acids 31 to 136 (106 residues), 135.2 bits, see alignment E=3.8e-44

Best Hits

Swiss-Prot: 96% identical to RS12_SPHAL: 30S ribosomal protein S12 (rpsL) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K02950, small subunit ribosomal protein S12 (inferred from 99% identity to sch:Sphch_1834)

MetaCyc: 76% identical to 30S ribosomal subunit protein S12 (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"SSU ribosomal protein S12p (S23e)" in subsystem Ribosomal protein S12p Asp methylthiotransferase or Ribosome SSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (136 amino acids)

>GFF3166 SSU ribosomal protein S12p (S23e) (Sphingobium sp. HT1-2)
VASGKVTEKVKGFMPTINQLVRKGRELQKTKSKVPAMDANPQKRGVCTRVYTTTPKKPNS
ALRKVAKVRLVNQREVITYIPGEGHNLQEHSVVLIRGGRVRDLPGVRYHVLRGVLDTQGV
KDRKQSRSKYGAKRPK