Protein Info for GFF3164 in Sphingobium sp. HT1-2

Annotation: Translation elongation factor G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 690 TIGR00484: translation elongation factor G" amino acids 1 to 688 (688 residues), 1108.2 bits, see alignment E=0 PF00009: GTP_EFTU" amino acids 9 to 281 (273 residues), 220.4 bits, see alignment E=4.8e-69 TIGR00231: small GTP-binding protein domain" amino acids 10 to 181 (172 residues), 107 bits, see alignment E=8.5e-35 PF22042: EF-G_D2" amino acids 308 to 390 (83 residues), 69.5 bits, see alignment E=6.3e-23 PF03144: GTP_EFTU_D2" amino acids 323 to 389 (67 residues), 58.5 bits, see alignment E=2.2e-19 PF14492: EFG_III" amino acids 402 to 475 (74 residues), 113.7 bits, see alignment E=1e-36 PF03764: EFG_IV" amino acids 477 to 595 (119 residues), 143.5 bits, see alignment E=8e-46 PF00679: EFG_C" amino acids 599 to 684 (86 residues), 106 bits, see alignment E=2.6e-34

Best Hits

Swiss-Prot: 90% identical to EFG_NOVAD: Elongation factor G (fusA) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K02355, elongation factor G (inferred from 95% identity to sch:Sphch_1832)

Predicted SEED Role

"Translation elongation factor G" in subsystem Translation elongation factor G family or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (690 amino acids)

>GFF3164 Translation elongation factor G (Sphingobium sp. HT1-2)
MARSHPLERYRNFGIMAHIDAGKTTTTERILYYTGKSYKIGEVHDGAATMDWMEQEQERG
ITITSAATTCIWNDHRLNIIDTPGHVDFTIEVERSLRVLDGAVAAFDGVAGVEPQSETVW
RQADKYKVPRMCFINKLDRTGADFYYCVQTIIDRLGATPAVLYLPIGAESDFKGLVDLVE
NRAIIWKDESLGAEFYYEEIPADLADKAAEYREKLIELAVEQDDAAMEAYLEGNLPDTAT
LKKLIRKGTLGHAFVPVLCGSAFKNKGVQPLLDAVVDYLPSPLDIEDVQGVKMDGETPDS
RPTSDDAPFSGLAFKIMNDPFVGSLTFLRIYSGTLTKGTYLNSVKDKKEKIGRMLLMHAN
SREDIDTAYAGDIVALAGMKETTTGDTLCAERQPIILERMEFPEPVIELSVEPKTKADQE
KMGVALNRLAAEDPSFRVSTDHESGQTIIKGMGELHLEILVDRMKREFKVEANVGAPQVA
YREYLKKAVDVDYTHKKQSGGTGQFGRIKVKLTPGERGSGFVFKDEIKGGNIPKEYIPAI
EKGMRETAATGSLIGFPIIDFEVLLYDGAYHDVDSSALAFEITGRAAMREAAQKSGITLL
EPVMKVEVVTPEEYLGDVIGDMNSRRGQIQGTDTRGNAQVVEAMVPLANMFGYVNSLRSF
TQGRANYSMIFSHYDEVPQNVADEVKAKMA