Protein Info for GFF3155 in Xanthobacter sp. DMC5

Annotation: putative methylmalonyl-CoA mutase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 27 to 547 (521 residues), 881.8 bits, E=1.3e-269
• PF01642: MM_CoA_mutase
  • amino acids 28 to 540 (513 residues), 756.2 bits, E=1.6e-231
• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 580 to 708 (129 residues), 196.3 bits, E=1.7e-62
• PF02310: B12-binding
  • amino acids 583 to 698 (116 residues), 68.6 bits, E=4.4e-23

Best Hits

Swiss-Prot: 76% identical to MUTB_STRCM: Methylmalonyl-CoA mutase large subunit (mutB) from Streptomyces cinnamonensis

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 96% identity to xau:Xaut_4731)

MetaCyc: 71% identical to methylmalonyl-CoA mutase large subunit (Propionibacterium freudenreichii shermanii)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (8/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• 3-hydroxypropanoate cycle (10/13 steps found)
• methylaspartate cycle (14/19 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (12/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (6/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.2

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (718 amino acids)

>GFF3155 putative methylmalonyl-CoA mutase large subunit (Xanthobacter sp. DMC5)
MSRIPNFAEIDWRAAAPAVPSAAAEAWTTPEGIPVKPLYGPADIEGLGYIDTYPGIAPFL
RGPYAPMYATQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPR
VAGDVGMAGVAIDSIYDMRTLFSGIPLDQMSVSMTMNGAVLPILALYVVAAEEQGVAASK
LSGTIQNDILKEFMVRNTYIYPPAPSMRIISDIFAFTSKEMPRFNSISISGYHMQEAGAT
QDLELAYTLADGVEYGRAGAAAGLPIDVFAPRLSFFWAIGMNFYMEVAKLRAARLLWTRL
MTDLGAKNPKSLPLRTHSQTSGWSLTAQDVFNNVMRTAIEAMAATQGQTQSLHTNALDEA
LALPTDFSARIARNTQILLQQESGTTRQIDLWGGSYFVEKLTADLAAKAWAHIQEVEALG
GMAKAIEAGIPKLRIEEAAATTQARIDGGAQTVVGVNKFKPQKDRLIDVLKVENATVRAA
QIEKLNRLKAERNPAEVAAKLEALTNGAAGNGNLLALAIEAARAKATVGEISDALEKVFG
RHRAEIRAISGVYKREASAMTDKVGKVQKLVEAFEHAEGRRPRILVAKVGQDGHDRGQKV
IASAFADLGFDVDIGPLFATPEEAARQAVENDVHVVGISSLAAGHLTLVPALKAALEAEG
RGDIMVVVGGVVPPQDYEALKAFGAEAIFPPGTVIADAAKELLGTLSHRLGHSLEAAE