Protein Info for GFF3151 in Sphingobium sp. HT1-2

Annotation: LSU ribosomal protein L14p (L23e)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 122 PF00238: Ribosomal_L14" amino acids 1 to 122 (122 residues), 186.4 bits, see alignment E=7.8e-60 TIGR01067: ribosomal protein uL14" amino acids 1 to 122 (122 residues), 191 bits, see alignment E=2.8e-61

Best Hits

Swiss-Prot: 91% identical to RL14_SPHWW: 50S ribosomal protein L14 (rplN) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K02874, large subunit ribosomal protein L14 (inferred from 98% identity to sjp:SJA_C1-06370)

MetaCyc: 77% identical to 50S ribosomal subunit protein L14 (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"LSU ribosomal protein L14p (L23e)" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (122 amino acids)

>GFF3151 LSU ribosomal protein L14p (L23e) (Sphingobium sp. HT1-2)
MIQMQSNLDVADNSGAKRVQCIKVLGGSKRRFAGVGDIIVVSVKEAAPRGKVKKGDVHRA
VIVRTAKDVRRADGSVIRFDGNAAVLINKNEEPIGTRIFGPVVRELRAKKHMKIISLAPE
VL