Protein Info for GFF3138 in Sphingobium sp. HT1-2

Annotation: Translation elongation factor LepA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 TIGR01393: elongation factor 4" amino acids 6 to 597 (592 residues), 975.5 bits, see alignment E=8.9e-298 PF00009: GTP_EFTU" amino acids 6 to 184 (179 residues), 182.9 bits, see alignment E=1.8e-57 TIGR00231: small GTP-binding protein domain" amino acids 9 to 178 (170 residues), 69.4 bits, see alignment E=3.1e-23 PF01926: MMR_HSR1" amino acids 18 to 135 (118 residues), 22.4 bits, see alignment E=3.9e-08 PF00071: Ras" amino acids 54 to 183 (130 residues), 22.2 bits, see alignment E=3.3e-08 PF03144: GTP_EFTU_D2" amino acids 208 to 278 (71 residues), 32.9 bits, see alignment E=2.6e-11 PF14492: EFG_III" amino acids 295 to 368 (74 residues), 27.1 bits, see alignment E=1.2e-09 PF00679: EFG_C" amino acids 402 to 479 (78 residues), 65.1 bits, see alignment E=1.7e-21 PF06421: LepA_C" amino acids 490 to 595 (106 residues), 173.5 bits, see alignment E=4.1e-55

Best Hits

Swiss-Prot: 87% identical to LEPA_NOVAD: Elongation factor 4 (lepA) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 94% identity to sch:Sphch_1804)

MetaCyc: 59% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (601 amino acids)

>GFF3138 Translation elongation factor LepA (Sphingobium sp. HT1-2)
MTDLSHIRNFSIIAHIDHGKSTLADRLIQRTGGLTDREMSAQVLDNMDIEKERGITIKAQ
TVRLDYTAKNGETYELNLMDTPGHVDFAYEVSRSLAACEGALLVVDAAQGVEAQTLANVY
QSIEHDHEIVPVLNKIDLPAAEPEKVRAEIEEVIGLDASEAVLASAKSGIGIDDILEAIV
TKIPAPKGDRNAPLEAMLVDSWYDPYLGVVILVRVVNGVIKKGQAIKFMIGGTEHLIDRV
GCMRPKIEALPELAAGEIGFITAQIKEIAQTRVGDTITTVKNGAAKPLPGFKEVQPVVFC
GLFPVDANDFEKLRESISKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSR
EYDLDLITTAPSVVYDMHMKDGRTLHLHNPADMPDPNHIEMIEEPWIEAVIYCPDEYLGS
ILKLCQDRRGIQKNLTYVGGRAQVTYELPLNEVVFDFYDRLKSISRGYASFDYHQIGTRE
GDLVKMSILVNNEPVDALSMIVHRSAAEARGRHMCERLKDLIPRHLFKIPIQAAIGGKVI
ARETIAAMRKDVTAKCYGGDITRKKKLLEKQKEGKKRMREYGSVQIPQEAFIAALRMGDE
N