Protein Info for GFF313 in Sphingobium sp. HT1-2

Annotation: Outer membrane vitamin B12 receptor BtuB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 618 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF07715: Plug" amino acids 40 to 147 (108 residues), 88 bits, see alignment E=8.6e-29 PF00593: TonB_dep_Rec_b-barrel" amino acids 167 to 592 (426 residues), 169.8 bits, see alignment E=2.9e-53 PF14905: OMP_b-brl_3" amino acids 422 to 601 (180 residues), 32.7 bits, see alignment E=6.9e-12

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 76% identity to sal:Sala_1004)

Predicted SEED Role

"Outer membrane vitamin B12 receptor BtuB" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (618 amino acids)

>GFF313 Outer membrane vitamin B12 receptor BtuB (Sphingobium sp. HT1-2)
MKMLGISLIALLAATPALAQDLAQDDDIVVTASGIAQPRDEVGQAITVIDADTIRTRQAV
DVVDLLATTPGVRFSRNGSTGGVTGVSLRGAETTQTLVLIDGVKVNDPSGIGDGYDFGHL
LTGNIRRIEVLRGSNSVVYGSQAIGGVVNVMTGAPSEGFEVNASADYGYSDTLNAKADVS
GTHGAVSGGVGVAYFNTDGISSVGRSFGGVEKDGYENIATHAKLKIAFTDSLSLDLRGYY
IHADLDYDSFFGAPADSSDVSKLDQYVGYAGINLALLDGKLTNRAAVTWMRNDRDYYFVR
GTDPDYGYSGTNLRFEYEGVYAPVDQAKLIFGYEHERPDYEYFGFGDIASAKANIDSVYA
LAVVKPLAGLAVTGGLRHDDHSQFGGATTFGANANYTPNAGATNIRLSYGEGFKAPSLYQ
LYDTYSGNSALRPERSKSYDIGFDQSLDGDKAMLSLTAFRRDTRNQINYDYATYTYGNLD
RTRAKGVEATLTLKPVEALTVAASYSYVDARDRSGGATDGNRLPRRAANAVSVSADYLWS
FGLSTGATLTMVGDSYDDTANTRRLDGYALAGLRASFPVGDHLEVYGRIDNLFDADYATA
YGYGTYGRSAYGGVRVRF