Protein Info for GFF3129 in Xanthobacter sp. DMC5

Annotation: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 987 transmembrane" amino acids 125 to 147 (23 residues), see Phobius details PF03710: GlnE" amino acids 68 to 305 (238 residues), 189.9 bits, see alignment E=8.4e-60 amino acids 579 to 818 (240 residues), 209.6 bits, see alignment E=8.2e-66 PF08335: GlnD_UR_UTase" amino acids 341 to 467 (127 residues), 104.9 bits, see alignment E=5.8e-34 amino acids 848 to 981 (134 residues), 30.4 bits, see alignment E=5.6e-11

Best Hits

Swiss-Prot: 84% identical to GLNE_XANP2: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 84% identity to xau:Xaut_3519)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (987 amino acids)

>GFF3129 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (Xanthobacter sp. DMC5)
MRWPTEPDSADRPPLALLMRETPVVADPKAAKIKLSQAIERLAPEARAALNALFVAAPCA
EQVCLGIAESSSFLIEVIRSEPERLLRVLDRPPHAHLDALIAECAAADGSEADVMRALRR
MRSEAALLIAVADMGGVFGLVEVTAALTDVADAAVRKALSFLLGEAVKAGRLTVPDPADP
QAGSGLAAIAMGKHGARELNYSSDIDLVVVYDREKAQLSDSVSASPFFVKITQGLVRLLQ
ERSADGYVLRVDLRLRPDPGSTQVALSTVSALDYYEREGATWERAAYIKARPVAGDAEVG
RQFLADLSPFVWRRVLDFQAIADVHAMKREIHAFRGHDVVAVEGHNVKLGRGGIREIEFF
VQTQQLIAGGRDPLLRTSRTLDALDALTAHRWIEPKVRDDLAEAYLFLRRVEHRIQMVAD
AQTHSLPESREAMEGFARFMGYPDRDAFAAALVARLTTVQSHYAHLFEDAPPPAVLDADL
LFPPDENDRPTLAALSRLGFKDPAAASGIVRRWLVGGPRALKTEPARAHLARIVPLMLEA
LSRGGDPDGALIAADRFFTELPGPHLLTALDRHPDLVRLLATILTAAPRLSETLARRPSL
TDALLDPAFFDVLPDEASLSEHLELLLDTAETDEEQFDRARRFRQEQHVLIGVRIASGTL
PAARAGEAYAKLAEVIIRALHRRVWVRFCETHGRIPGAETAVLAMGKLGGREMTAGSDLD
LIVLYDFDPEADGTSDGPRPLTGAQYFARFTQRLVTALTSLTNAGKLYDVDLRLRPSGRS
GPLATRLSSFATYQQAEAWTWEHMALTRARVIAATPEFGGIVRDVIGGIMGQARDRRRLA
GDILDMRQAIAAEKGEDDRWNLKHAAGGQVDVEFLAQYLVLAHAHDHPEIVDTATARVLA
TAERLALLEPEDAHVLLRACRLYQNLTQVLRLSVDAHVVPQDASPALRALLARAGEMPDF
ATLEADLFETEQKVRAIFERVLETAAE