Protein Info for PS417_15955 in Pseudomonas simiae WCS417

Annotation: secretion protein HylD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 37 to 354 (318 residues), 186.3 bits, see alignment E=3.4e-59 PF16576: HlyD_D23" amino acids 59 to 261 (203 residues), 37.8 bits, see alignment E=2.6e-13 PF13533: Biotin_lipoyl_2" amino acids 67 to 107 (41 residues), 23.4 bits, see alignment 8.3e-09 PF13437: HlyD_3" amino acids 172 to 258 (87 residues), 33.1 bits, see alignment E=1.7e-11

Best Hits

KEGG orthology group: None (inferred from 95% identity to pfs:PFLU3645)

Predicted SEED Role

"RND efflux membrane fusion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TYD6 at UniProt or InterPro

Protein Sequence (368 amino acids)

>PS417_15955 secretion protein HylD (Pseudomonas simiae WCS417)
MHPLPLRLLTPLALLIILNGCNSKADTAAETPLPRPVLAAKVEAAGTQQSAYTGVVAART
ESDLGFRVSGKVIERKVDPGQHVSRGDTLLVLDIGDFELALRSAKNRVHAAQAQLRQRRD
DENRYQRLASTGAVSRQIFDQSATLLRVAEAELASAQSDASQIENRRTYSVLKADGDGII
TDVRVDRGQVVAEGQIVARLAHDGAREAIVNLPENQRDQAAQKALAFPFGAPDQAVTATL
RELSASADPTTRTYRARYVLHGAVDRFALGSTITVRLQGNGQAQQTRVPIGALLDSGQGT
GVWLIGADDKVSFAPVKVASLGQEDALLDSGVSPGQVIVALGAHLLHSGDPVRLLPAQVL
ALNRKQDN