Protein Info for GFF3118 in Variovorax sp. SCN45

Annotation: Membrane alanine aminopeptidase N (EC 3.4.11.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 897 TIGR02414: aminopeptidase N" amino acids 17 to 894 (878 residues), 1150.8 bits, see alignment E=0 PF17900: Peptidase_M1_N" amino acids 108 to 191 (84 residues), 49.1 bits, see alignment E=1.5e-16 PF01433: Peptidase_M1" amino acids 231 to 443 (213 residues), 170.2 bits, see alignment E=1.1e-53 PF11940: DUF3458" amino acids 458 to 562 (105 residues), 93.2 bits, see alignment E=2.2e-30 PF17432: DUF3458_C" amino acids 566 to 895 (330 residues), 385.4 bits, see alignment E=5e-119

Best Hits

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 95% identity to vap:Vapar_3793)

Predicted SEED Role

"Membrane alanine aminopeptidase N (EC 3.4.11.2)" (EC 3.4.11.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.2

Use Curated BLAST to search for 3.4.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (897 amino acids)

>GFF3118 Membrane alanine aminopeptidase N (EC 3.4.11.2) (Variovorax sp. SCN45)
MLQRDAQGQPIAIRREDYAAPAYWIDTVDLTFDLDPAKTRVLNRMQMRRNPEVPVQPLRL
DGDELNLARVLVNGQGASFRMEGDQLVIDGLPDAFELELFTTCCPIKNTKLMGLFVSEDT
FFTQCEAEGFRRITYFLDRPDVMASYTVTLRAAKAAYPVLLSNGNLVEQGELPEGRHFAK
WVDPFKKPSYLFALVAGKLVAREQRIKARSGKEHLLQVYVRAGDLDKTEHAMNSLINSVL
WDEARFGLPLDLDRFMIVATSDFNMGAMENKGLNIFNTKYVLANQATATDADYSNIESVV
GHEYFHNWSGDRVTCRDWFQLSLKEGLTVFRDQEFSQDLCADASARAVKRIEDVRVLRTA
QFPEDAGPMAHPVRPDSYIEISNFYTVTIYEKGAEVVRMMQTLVGRKGFEKGITLYFERH
DGQAVTCDDFAQAIADANPESELARLLPQFKRWYSQAGTPRLAAHGVYDPANRSYTLSVV
QSCPPTPGQPAKEPFVIPLNVGLLDANGRELPLQLEGESEVTRGTRTLVLSRAGEQITFV
GLDAEPVPSILRGFSAPVILDFEYTDAQLLTLLANDPDPFNRWEAGQRLGLRAALQGIGA
LATDTTPVLNDAYIDAMRSVLRNPQLDAAFKELVLTLPSETYIAEQLEVVDPQRVHLVRE
AMRAQLATALFADWQQAYEDNHDTGAYSPDPTSSGRRALAGMALNFLCLAARSSGDVVWP
GKTLQRFKDAGNMTDRFNALNALVSSGHTLAAQALARFHAIFKDEALVIDKWFSLQAGAS
DRGGDVLPLVKQLMKHPDFSLKNPNRARSVIFSYCSANPGAFHRPDAAGYVFWSERVIEL
DAINPQVAARLARALDRWSKLAEPYRSAAREAIARVAAKPDLSKDTHEVVTRALAGN