Protein Info for PS417_15950 in Pseudomonas simiae WCS417

Annotation: major facilitator transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 401 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 42 to 65 (24 residues), see Phobius details amino acids 77 to 95 (19 residues), see Phobius details amino acids 101 to 123 (23 residues), see Phobius details amino acids 135 to 157 (23 residues), see Phobius details amino acids 164 to 184 (21 residues), see Phobius details amino acids 216 to 244 (29 residues), see Phobius details amino acids 250 to 268 (19 residues), see Phobius details amino acids 280 to 302 (23 residues), see Phobius details amino acids 311 to 332 (22 residues), see Phobius details amino acids 340 to 363 (24 residues), see Phobius details amino acids 370 to 390 (21 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 10 to 388 (379 residues), 207.1 bits, see alignment E=2.9e-65 PF07690: MFS_1" amino acids 20 to 354 (335 residues), 118.8 bits, see alignment E=2.7e-38 PF00083: Sugar_tr" amino acids 47 to 191 (145 residues), 42.8 bits, see alignment E=3.4e-15

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 91% identity to pfs:PFLU3644)

Predicted SEED Role

"Multidrug resistance transporter, Bcr/CflA family" in subsystem Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UMB7 at UniProt or InterPro

Protein Sequence (401 amino acids)

>PS417_15950 major facilitator transporter (Pseudomonas simiae WCS417)
MTNAPAPSRSLLFLLVALTALGEVSTQLIIPGLGAIEQALAAPAGSSLMALSAFVAAFGL
GQLLFGPLSDRIGRRPVLIGGLVLYGLATLSMLLVDDIEHFIAARVLQGLGACAALVLAR
TVVRDVWKAEAGPALALTMIGMLYAIVVAPMVGGLLIKWLGWRAPIMLALAIGSVVLLVV
LLFFRESNHYLDPKAGHWRSLGGQYLDLLRGRQYRAFAVALACTYGAMFAVIAGSSAVFI
NLLGFSSLEYGINFGLIVSMLIVGSTYTRRNIMRLGPQRIVTMGVAMVAAGGVSAVVIYA
LFGLSVLGLDIPIALVTLGGGLVLPGSVTGGVMPNAHRAGLAAGLMGFAQMFGATCSGVL
LSLLRDGSATPMIVIQACFAATACVAFHLLRQRQANGLSYT