Protein Info for GFF3107 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to TRPG_PSEAE: Anthranilate synthase component 2 (trpG) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K01658, anthranilate synthase component II [EC: 4.1.3.27] (inferred from 86% identity to pna:Pnap_3652)MetaCyc: 65% identical to anthranilate synthase beta subunit (Pseudomonas aeruginosa PAO1)
Anthranilate synthase. [EC: 4.1.3.27]
Predicted SEED Role
"Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85)" (EC 2.6.1.85, EC 4.1.3.27)
MetaCyc Pathways
- superpathway of aromatic amino acid biosynthesis (18/18 steps found)
- superpathway of L-tryptophan biosynthesis (13/13 steps found)
- superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
- L-tryptophan biosynthesis (6/6 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of chorismate metabolism (43/59 steps found)
- 4-aminobenzoate biosynthesis I (2/2 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- 4-hydroxy-2(1H)-quinolone biosynthesis (3/5 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- acridone alkaloid biosynthesis (1/4 steps found)
- superpathway of candicidin biosynthesis (4/11 steps found)
- superpathway of quinolone and alkylquinolone biosynthesis (3/10 steps found)
- chloramphenicol biosynthesis (1/9 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Folate biosynthesis
- Phenylalanine, tyrosine and tryptophan biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.85, 4.1.3.27
Use Curated BLAST to search for 2.6.1.85 or 4.1.3.27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (196 amino acids)
>GFF3107 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) (Hydrogenophaga sp. GW460-11-11-14-LB1) MKPIRLLMVDNYDSFTFNIVQYFGELGAEVSVFRNDEITLEAIEARLQAGQLDRLVISPG PCSPAEAGISVAAIQHFAGKLPILGVCLGHQSIGAAFGGKIVRAQELMHGKTSQITTTRQ GVFANLPEQFTVNRYHSLAIERASCPDCFDVTAWTPDGEIMGVKHKTLAIEGVQFHPESI LTEHGHAMLRNFLEQA