Protein Info for GFF3104 in Sphingobium sp. HT1-2

Annotation: Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1190 PF02811: PHP" amino acids 52 to 172 (121 residues), 28.3 bits, see alignment E=5.1e-10 TIGR00594: DNA polymerase III, alpha subunit" amino acids 265 to 1113 (849 residues), 438.3 bits, see alignment E=3.3e-135 PF07733: DNA_pol3_alpha" amino acids 380 to 638 (259 residues), 264.2 bits, see alignment E=3.5e-82 PF17657: DNA_pol3_finger" amino acids 641 to 815 (175 residues), 171.3 bits, see alignment E=2.9e-54 PF14579: HHH_6" amino acids 889 to 991 (103 residues), 64 bits, see alignment E=3.1e-21 PF01336: tRNA_anti-codon" amino acids 1079 to 1152 (74 residues), 29.1 bits, see alignment 2e-10

Best Hits

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1190 amino acids)

>GFF3104 Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7) (Sphingobium sp. HT1-2)
MSGRRPPPDPAKPRSIDRRELNAIEAAREKTKPAPPRPAPAEPPFAELVSISNYSFLHGA
SHPADLVGEAIALGLKAIGLADRNSVAGVVRAYVALRDAPEKARAKLLEDKKARGEPESL
TPEEEAGCETDLRLIVGARLIFVDGTPDIIVYPASRQGWGKLTRLLTKGNIKAIKGDCIL
HFADLLDYLDDWRMIILPLSTAQAQQEHDAPPDYAFEDEAIPVPSRHLRLVPPASPPSWQ
EAARQLQDRMPGRVWLGLSMRYQGRDARHVAMMQAVSRDIGIPLIATNDILYATSGTRPL
QDVLTCIREGVTLKTAGTRLQANAERHMKSPTEMARLFSACPDALAETIRFIDGIDFTLK
DLAYEYPHEPVPAGWSPDAWLEDMVRRRARRRYGRQLPPKVRALIGQELRLIRRQNYAYY
FLTVHDLVRFARAQKPPILCQGRGSAANSIVCFLLGVTSIDPMEHDLLFSRFVSEERNEP
PDIDVDFEHERREEVMQYIYRRYGRARAGIAATVIHYRSRSALREVAKVLGLSEDVVMRL
SATVWGSYSGDLGDARFVDAGFALDNGEIQRLRTLVQQLIRAPFPRHLSQHVGGFVLTQD
RLDETVPIHNAAMADRSFIEWDKDDIDALNLMKVDVLALGMLSCIRRAYALMNQHGLGRH
DLTVDLQSRDPAVYDMLCKGDSIGVFQVESRAQINMLPRLKPRNLYDLTVQVAIVRPGPI
EGDMVHPYLRRRCGEEKVDYPSPGTDFGPPDELEKLLKDTYGVPLFQEQAMKLAIVAAGF
SSSEANQLRRAMATFRNVGTIHHFREKMINGMTARGYEAEFAARCFKQIEGFGSYGFPES
HALSFARLVYVSAWIKCHQPAIFACALLNSQPMGFYAPAQLVRDAREHGVAIHDVDVNMS
GWDNGLEPLLRSRHADKGEGTGRALALRLGLRQVDGFREQWAAQMAAARAQGGLFTSIED
MARRAGLPARALRLLADADACRSLGQDRRTALWEARRTPSDELPLFAAARRHDRAARELG
EEADAMLPAMPLAEQVMDDYAVTRLSLKGHPMQFLRPVFAAEGVLSCAQTSAAKNGARVR
TAGVVLVRQRPGKGNAVFITIEDETGITNILLWARLFELQRRPVMASRLMLVEGEVQRSK
EGVVHLMASRIHDRTADLNRLSQSEPPKQAAPAARHPRDVRILPKSRDFH