Protein Info for GFF310 in Sphingobium sp. HT1-2

Annotation: ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 401 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details PF04348: LppC" amino acids 66 to 244 (179 residues), 45.8 bits, see alignment E=5.6e-16 PF13458: Peripla_BP_6" amino acids 70 to 385 (316 residues), 92.7 bits, see alignment E=5.5e-30 PF01094: ANF_receptor" amino acids 106 to 244 (139 residues), 27.6 bits, see alignment E=2.4e-10

Best Hits

KEGG orthology group: None (inferred from 80% identity to sch:Sphch_1711)

Predicted SEED Role

"ABC-type branched-chain amino acid transport systems, periplasmic component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (401 amino acids)

>GFF310 ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate) (Sphingobium sp. HT1-2)
MTETDAPRQANMFAAMKRSARLLFVASTIMLAACQSIVPKGPGPAQPTGPAKPTGPEVTQ
GLPTDTARHRVALLVPTSGPNAGVGQSIANATTLALMDIKTDKVRITTYDTATGAAAAAN
RALADGNRLILGPLLADDARIVGPIAAKANVPVISFSNDTSVAGSNIFLMGYTPNQSIER
VIGFAREKGLSNFGALVPRGTYGERAGNALLRAVEQAGGTVVSMQTFDRTPASITAAVKK
LQASSSYDALLIADSGRVALQVAPIVRKNGGANARLLGTEIWNTEESLAASPVLRGAWYA
SVSDGLYRQLATKYRTRYGSAPFRLSSMGYDAVLLTVRIAQDWKPGAPFPTARLRDPGGF
AGIDGAFRFNRNGIAERALEVSEVGAGTISVVDPAPRGFGN