Protein Info for GFF3092 in Variovorax sp. SCN45

Annotation: Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 23 to 416 (394 residues), 338.4 bits, E=3.8e-105
• PF08245: Mur_ligase_M
  • amino acids 44 to 216 (173 residues), 38.2 bits, E=1.5e-13
• PF02875: Mur_ligase_C
  • amino acids 284 to 408 (125 residues), 67.3 bits, E=2.4e-22

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 94% identity to vpe:Varpa_4356)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of chorismate metabolism (42/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (431 amino acids)

>GFF3092 Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17) (Variovorax sp. SCN45)
METLNDWLARAEQLHPKNIELGLERAKEMAQRLGLRFGCPVITVAGTNGKGSTCAMLESI
LTHAGYRTAVFTSPHLVRFEERLRLKGEAVDASKLIASFEVVEKARGDMPLTYFEFTTLG
ILHCMIEEKPDVAILEVGLGGRLDAVNIIDTDCAVITSIDLDHMEYLGPDRESIGFEKAG
IMRAGKPVVVSDPMPPQSVIDHANAIGADLWRFGTDFNVSGDKQQWGWSGRGRRYSGLAY
PALRGANQLINAAGVLAALEALRPQLPITAQAVRNGLAMVELPGRFQIVPGEPALVLDVA
HNAHAVAALAENLDAMGFYPTTHGVFGVMADKDLAPILARIGPLIDRWYFTDLPTSRAAK
AADLLASWQAQNTRTDASGSVHAGPMEALRAAIEQAGPADRIVVFGSFFTVGGVLENGTP
RLQAKHLLPGG