Protein Info for GFF3086 in Sphingobium sp. HT1-2

Annotation: Uncharacterized inner membrane protein RarD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 45 to 67 (23 residues), see Phobius details amino acids 74 to 94 (21 residues), see Phobius details amino acids 100 to 123 (24 residues), see Phobius details amino acids 132 to 149 (18 residues), see Phobius details amino acids 152 to 169 (18 residues), see Phobius details amino acids 181 to 201 (21 residues), see Phobius details amino acids 213 to 234 (22 residues), see Phobius details amino acids 241 to 263 (23 residues), see Phobius details amino acids 270 to 292 (23 residues), see Phobius details PF00892: EamA" amino acids 10 to 145 (136 residues), 48.5 bits, see alignment E=5.6e-17 amino acids 155 to 284 (130 residues), 29.5 bits, see alignment E=4.1e-11 TIGR00688: protein RarD" amino acids 10 to 261 (252 residues), 213.1 bits, see alignment E=2.5e-67

Best Hits

KEGG orthology group: K05786, chloramphenicol-sensitive protein RarD (inferred from 73% identity to sch:Sphch_0290)

Predicted SEED Role

"Protein rarD"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (300 amino acids)

>GFF3086 Uncharacterized inner membrane protein RarD (Sphingobium sp. HT1-2)
VTIAESEARKGVIAAIGTYSIWGMLPLFFRLLHHVDPIEIVTQRVIWSVILILVLLAARK
GLAAFVAALRTPRLVLPLAASAVMIAINWLVYVWAVNDGHVVACSLGYFLNPLVSVGLGV
VILKERLRRGQMLAIAIAAIGVAILAAAALTTLWISLALAISFALYGLIRKLTPVAPMTG
LGVETLLLVAPAIGYLIWLAGHGGISFGQDVPTTGLLILAGGVTTVPLVLFATAAQRLPL
ATLGLLQYLAPTLQFLCGVVLLGETLSKGQMLSFGLIWLGLILFASDSYAAARRNRLATA