Protein Info for PS417_15740 in Pseudomonas simiae WCS417

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 215 PF00072: Response_reg" amino acids 9 to 120 (112 residues), 105.9 bits, see alignment E=2e-34 PF04545: Sigma70_r4" amino acids 152 to 195 (44 residues), 26.5 bits, see alignment 5.6e-10 PF00196: GerE" amino acids 153 to 207 (55 residues), 72 bits, see alignment E=3.8e-24

Best Hits

Swiss-Prot: 51% identical to NARL_ECO57: Nitrate/nitrite response regulator protein NarL (narL) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 84% identity to pba:PSEBR_a3152)

MetaCyc: 51% identical to DNA-binding transcriptional dual regulator NarL (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Nitrate/nitrite response regulator protein" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UGK1 at UniProt or InterPro

Protein Sequence (215 amino acids)

>PS417_15740 hypothetical protein (Pseudomonas simiae WCS417)
MNTAIRHRILLVDDHPMMRHGMRQMLELEDDLHVVGEAGNGQEALDQIATLQPDLVLLDN
NMPHMNGLETLRRLRAMNYAGKVLLFTVSDAEDDIRDALRLDANGYLLKDMEPELVVEFI
RDALQGALVISPGLTRVLAQALRSPQPHAAVELTERERQVLRTIAGGYSNKVIGHKLGIT
EGTVKVHVKNVLHKLGLRSRVEAAVWAMEHLRQAG