Protein Info for PS417_15735 in Pseudomonas simiae WCS417

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 signal peptide" amino acids 12 to 14 (3 residues), see Phobius details transmembrane" amino acids 15 to 37 (23 residues), see Phobius details amino acids 157 to 177 (21 residues), see Phobius details PF13675: PilJ" amino acids 34 to 128 (95 residues), 43.5 bits, see alignment E=6.2e-15 PF00672: HAMP" amino acids 181 to 227 (47 residues), 48.8 bits, see alignment 1.4e-16 PF07730: HisKA_3" amino acids 391 to 456 (66 residues), 47.6 bits, see alignment E=4.2e-16 PF02518: HATPase_c" amino acids 499 to 587 (89 residues), 42.1 bits, see alignment E=2.1e-14

Best Hits

KEGG orthology group: K07673, two-component system, NarL family, nitrate/nitrite sensor histidine kinase NarX [EC: 2.7.13.3] (inferred from 78% identity to pba:PSEBR_a3151)

Predicted SEED Role

"Nitrate/nitrite sensor protein (EC 2.7.3.-)" in subsystem Nitrate and nitrite ammonification (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U2Q2 at UniProt or InterPro

Protein Sequence (600 amino acids)

>PS417_15735 histidine kinase (Pseudomonas simiae WCS417)
MFDWLRSSLPARAGLAVILIAILALASSLSAGLIAWFSQGDAAAINTAGSVRMETYHLSW
KLAAGARPDEVAEVSQSLQRRLDSQSLKAVLEDGPRSALLQSYQQIQRRWNDELRPAVMR
GDAVLFQVSAPAFVEQLNQFVSLLQQQSEHKQSWQQAIQGLALFSTLIILLIGLYELQYG
VVTPLQELVHATRRFRDGNYQTRVNHRSEDELGQLALSFNAMAETIEASHRTLEAQVQLK
TLNLQQANAALELLYQSSRSLATRLANAEGLDELIRRFQKRLPGLRLSLCLQGHFLAPAQ
QMLALHGEHSRNVCAIGDCATCERHHAAPPEVFSISNQGAELGELKAHFLDGHPPQDWEK
QLIQALANLIGTSLSLKRQREQDHRLLLLEERTIIARELHDSLAQALSYMKLQVSRMQTL
IRRGEPVETLACVTSELRDGLNNAYRQLRELLTTFRLQIHDAGLVQELRETAEEFSRRGE
FQVHLHVDALAFQLSASEQIHVLQITREALSNCLRHAHAQNAWLQLRQVGETVSLCIEDD
GRGFGGDVDQREHHGLTIMDERARSLHGHLDISPREPQGTRVQLRFQPEFLGHSLQGTAS