Protein Info for PS417_15690 in Pseudomonas simiae WCS417

Annotation: ribonucleoside-triphosphate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 662 PF03477: ATP-cone" amino acids 15 to 97 (83 residues), 63.3 bits, see alignment E=2.6e-21 TIGR02487: anaerobic ribonucleoside-triphosphate reductase" amino acids 126 to 656 (531 residues), 455 bits, see alignment E=2e-140 PF13597: NRDD" amino acids 127 to 657 (531 residues), 580.2 bits, see alignment E=5e-178

Best Hits

KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 89% identity to pba:PSEBR_a3142)

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UGI4 at UniProt or InterPro

Protein Sequence (662 amino acids)

>PS417_15690 ribonucleoside-triphosphate reductase (Pseudomonas simiae WCS417)
MQSTLTPLTSTHLHKRDGSLAPFDAEKIRQALIAAGTATAEFQANDADLLLGAVLARLRG
IEQLDVELIQDSVERVLMDAGYFQSMRAYIVYREQHGRMRRDRKTLVEVATSMNEYLDRE
DWRVQANANQGYSLGGLVLNVAGKVTANYWLDEVYSEQIGRAHREADLHIHDLDMLAGYC
AGWSLRSLLHEGLNGVPGRVEAGPPKHLSSALGQMVNFLGTLQNEWAGAQAFSSFDTYLA
PYVRKDQLSYPQVRQAVQEFIYNLNVPSRWGTQTPFTNLTFDWVCPEDLREQVPVIGGEE
MPFAYGDLQAEMELINRAYIEVMQAGDAKGRVFTFPIPTYNITHDFPWDSDNADRLFEMT
ARYGLPYFQNFLNSDMQPNQVRSMCCRLQLDVRELLKRGGGLFGSAEQTGSLGVVTINCA
RLGYLFKGDTSGLLQRLDSLMEMAMESLEVKRKVIQHHMDAGLYPYTKRYLGTLRNHFST
IGLNGMHEMLRNFSADEEGMHTEQGRAFALKLLDHVRATLLRFQEDTGHLYNLEATPAEG
TTYRFAKEDRKRYPNILQAGSDIAPYYTNSSQLPVGFTQDPFEALELQDELQCKYTGGTV
LHLYMAEQISSTQACKQLVRKALGRFRLPYLTVTPTFSICPVHGYLAGEHEFCPKCDEVL
AL