Protein Info for GFF3065 in Xanthobacter sp. DMC5

Annotation: Bicyclomycin resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 transmembrane" amino acids 29 to 51 (23 residues), see Phobius details amino acids 73 to 91 (19 residues), see Phobius details amino acids 103 to 121 (19 residues), see Phobius details amino acids 127 to 149 (23 residues), see Phobius details amino acids 161 to 184 (24 residues), see Phobius details amino acids 190 to 209 (20 residues), see Phobius details amino acids 242 to 261 (20 residues), see Phobius details amino acids 276 to 298 (23 residues), see Phobius details amino acids 310 to 329 (20 residues), see Phobius details amino acids 335 to 354 (20 residues), see Phobius details amino acids 365 to 390 (26 residues), see Phobius details amino acids 398 to 419 (22 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 36 to 416 (381 residues), 207.7 bits, see alignment E=1.9e-65 PF07690: MFS_1" amino acids 41 to 387 (347 residues), 120.4 bits, see alignment E=8.6e-39 PF00083: Sugar_tr" amino acids 74 to 147 (74 residues), 24.1 bits, see alignment E=1.7e-09

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 85% identity to xau:Xaut_3226)

Predicted SEED Role

"MFS family multidrug efflux protein, similarity to bicyclomycin resistance protein Bcr"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (429 amino acids)

>GFF3065 Bicyclomycin resistance protein (Xanthobacter sp. DMC5)
MSQSHRAPAPASASASRPRGPAPGQPGAVGPGFVEFVALIALLMGMTAFSIDNLLPAFGA
IRADFALENANHVQVMVYAYLIGIGGAQFFYGPISDVLGRRPILFAGLAVYALGSLIALF
TRDFNVLIVARLIQGMGAAAGRVLAIAIVRDRFEGRDMARIMSLSMMVFLTVPIIAPALG
SLILVFGSWHLIFVAMLLLALALFAWFGLRMPETLHPEYRIPFSMRSIGKAAMLTLTTRR
SVGYAVAIGLMMGSLMAYVGSASQVFQTDVYRLGNLFPIAFAAVAGIMAVASFLNASLVH
RLGMRRLSHAGILGFIAAGGLMVASSVIWNGKPPLVLFCGLVAAGQFLFALTVPNFNSMA
MEPLGAVAGTASSFIGGFTTLLAAVLGFVVGSNFDGTVLPLALGYLVLGAIALLFVLWAE
KGKLFGHQP