Protein Info for PS417_15680 in Pseudomonas simiae WCS417

Annotation: radical SAM protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 222 TIGR02495: anaerobic ribonucleoside-triphosphate reductase activating protein" amino acids 1 to 185 (185 residues), 206.7 bits, see alignment E=1.1e-65 PF13353: Fer4_12" amino acids 14 to 73 (60 residues), 30.8 bits, see alignment E=3.4e-11 PF04055: Radical_SAM" amino acids 18 to 149 (132 residues), 57.8 bits, see alignment E=1.7e-19

Best Hits

KEGG orthology group: None (inferred from 77% identity to pba:PSEBR_a3141)

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4)" in subsystem Ribonucleotide reduction (EC 1.97.1.4)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.97.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U7S6 at UniProt or InterPro

Protein Sequence (222 amino acids)

>PS417_15680 radical SAM protein (Pseudomonas simiae WCS417)
MVPLTTLDYPGMLACVLFCQGCAWRCRYCHNPDLIPPRASTQVDWRRVLLFLQRRRDLLD
AVVFSGGEPTLQEGLPAAMDEVRAMGFHIGLHSAGIKPAGFAHALRHADWVGFDVKALAE
DCQSITQVKGSGAANWRSLDILLASGMDYECRTTVHWHLIDPARLLRLAQRLQARGVQRF
AVQMVRTASMLDTQLPSSPAQAALPELWGRLRELFPAFVLRG