Protein Info for GFF3055 in Sphingobium sp. HT1-2

Annotation: Fumarate hydratase class II (EC 4.2.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00979: fumarate hydratase, class II
  • amino acids 5 to 461 (457 residues), 690.5 bits, E=5.2e-212
• PF00206: Lyase_1
  • amino acids 12 to 343 (332 residues), 346.1 bits, E=2.2e-107
• PF10415: FumaraseC_C
  • amino acids 409 to 461 (53 residues), 78.6 bits, 3.7e-26

Best Hits

Swiss-Prot: 64% identical to FUMC_BRUME: Fumarate hydratase class II (fumC) from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

KEGG orthology group: K01679, fumarate hydratase, class II [EC: 4.2.1.2] (inferred from 78% identity to sch:Sphch_0310)

MetaCyc: 60% identical to fumarase subunit (Ascaris suum)
Fumarate hydratase. [EC: 4.2.1.2]

Predicted SEED Role

"Fumarate hydratase class II (EC 4.2.1.2)" in subsystem TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• mixed acid fermentation (13/16 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• methylaspartate cycle (13/19 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• reductive TCA cycle I (6/11 steps found)
• incomplete reductive TCA cycle (3/7 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• reductive TCA cycle II (6/12 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.2

Use Curated BLAST to search for 4.2.1.2

Protein Sequence (461 amino acids)

>GFF3055 Fumarate hydratase class II (EC 4.2.1.2) (Sphingobium sp. HT1-2)
MSATRTETDSIGAIEVPADAYWGAQTQRSIENFPFGATERMPIGIVHAQAIVKQAAARVN
AKHGLDAKIVAGIENAAQQIVSGALDDQFPLVIWQTGSGTQTNMNVNEVIAGYANEQLAG
TRGGKSPVHPNDHVNMSQSSNDSFPTALHVATVLATRDKLIPALEKLTGALTAKAEGWGH
IVKIGRTHTQDATPLTLGQEFSGYAAQLVSSKARIEGVLNGNIRKLAIGGTAVGTGLNAP
DGWADDMTAAISDIAGTSFESAPNKFEQLAAKDGLVFFSGALNTLAVALTKIANDIRFLG
SGPRSGLGELDLPANEPGSSIMPGKVNPTQCESLTMVAAQVIGNHQAVTVGGMQGHFELN
VFMPLIGANVLRSIHLLSVGMESFADRCVEGMQANEGRIAELVERSLMLVTALAPEIGYD
NAATIAKHAHKKGLTLKQAGLELGLVDEPTFDRLVRPENMV