Protein Info for GFF3052 in Sphingobium sp. HT1-2

Annotation: Guanylate kinase (EC 2.7.4.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03263: guanylate kinase
  • amino acids 17 to 195 (179 residues), 234.7 bits, E=2.9e-74
• PF00625: Guanylate_kin
  • amino acids 18 to 196 (179 residues), 165.7 bits, E=4.8e-53

Best Hits

Swiss-Prot: 69% identical to KGUA_SPHAL: Guanylate kinase (gmk) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K00942, guanylate kinase [EC: 2.7.4.8] (inferred from 93% identity to sjp:SJA_C1-15600)

MetaCyc: 43% identical to guanylate kinase (Saccharomyces cerevisiae)
Guanylate kinase. [EC: 2.7.4.8]; T(2)-induced deoxynucleotide kinase. [EC: 2.7.4.8, 2.7.4.12, 2.7.4.13]

Predicted SEED Role

"Guanylate kinase (EC 2.7.4.8)" in subsystem MLST or Purine conversions (EC 2.7.4.8)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (8/9 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• superpathway of purine nucleotide salvage (11/14 steps found)
• dZTP biosynthesis (4/5 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.12 or 2.7.4.13 or 2.7.4.8

Protein Sequence (221 amino acids)

>GFF3052 Guanylate kinase (EC 2.7.4.8) (Sphingobium sp. HT1-2)
MADNIPTDTPDFKRRGVLFVLSSPSGAGKSTIARKLLACEPDLSMSVSATTRTIRPGEVD
GKDYHFVDLEEFRRMANDHEFLEWAHVFGQRYGTPRAPVEAMLKSGKDVLFDIDWQGAQQ
LHQIAGGDVVRIFILPPSMEELEKRLRGRATDSNEVIEGRMARAAGEIAHWDGYDYVLCN
VDVEECFERVRTILHAERMKRSRQTGLIGFIRRLSRYHQED