Protein Info for GFF3047 in Sphingobium sp. HT1-2

Annotation: Two-component oxygen-sensor histidine kinase FixL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 381 TIGR00229: PAS domain S-box protein" amino acids 16 to 141 (126 residues), 103.9 bits, see alignment E=3.4e-34 PF13188: PAS_8" amino acids 22 to 73 (52 residues), 23.2 bits, see alignment 1.5e-08 PF00989: PAS" amino acids 22 to 130 (109 residues), 71.4 bits, see alignment E=1.9e-23 PF08448: PAS_4" amino acids 26 to 135 (110 residues), 36.9 bits, see alignment E=1.1e-12 PF13426: PAS_9" amino acids 28 to 133 (106 residues), 44.9 bits, see alignment E=3.8e-15 PF00512: HisKA" amino acids 157 to 224 (68 residues), 37.4 bits, see alignment E=6.3e-13 PF02518: HATPase_c" amino acids 271 to 376 (106 residues), 74.1 bits, see alignment E=3.6e-24

Best Hits

KEGG orthology group: K14986, two-component system, LuxR family, sensor kinase FixL [EC: 2.7.13.3] (inferred from 77% identity to sch:Sphch_3367)

Predicted SEED Role

"Two-component oxygen-sensor histidine kinase FixL" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (381 amino acids)

>GFF3047 Two-component oxygen-sensor histidine kinase FixL (Sphingobium sp. HT1-2)
MAFNDGDPQDNATSREQALLGSILATVPDALIVIDATGHIVSFSPAAQRMFQYAEADVLG
RNVAMLMPSPDRERHDSYLQHYHDTGEKRIIGIGRLTTARRRDGSTFPIELAVGEVQDHG
EKLFTGFVRDLTDRQKAERRLQDLQAELSHAARVTAMGTLAAALAHELNQPLTAIANYME
AGRDLLRTDAPVDRDLLGEAMDEAARQAMRAGQIIRSLREFIRRGEADRQAEPAATLLAE
GVALAFIGIDSRGIDMDIVVDPRVGRVIANRVQVQQVIINLVRNAVEAMDGRNRRILRLS
AVPAEDGQVELIIADSGPGLDPAVARSLFTPFSTTKPAGMGVGLSISRTIVEGHGGRIWA
DASHWGGVAFHFTLDSADRLP