Protein Info for PS417_15585 in Pseudomonas simiae WCS417

Annotation: DNA repair nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 470 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF00817: IMS" amino acids 18 to 150 (133 residues), 34.9 bits, see alignment E=7e-13

Best Hits

KEGG orthology group: K14161, protein ImuB (inferred from 93% identity to pfs:PFLU3603)

Predicted SEED Role

"DNA polymerase-like protein PA0670" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1TP47 at UniProt or InterPro

Protein Sequence (470 amino acids)

>PS417_15585 DNA repair nucleotidyltransferase (Pseudomonas simiae WCS417)
MRWVCIVFPQLALDGVQRAHPEPEQPLVLLAGTPQRRVLQTVNDAARALGLRPGQSLTAA
HALAKTFASAEYDPAEIERYQQFLAAWAYQFSSQVSLYYPRALLFEIESSLGLFGPWPRF
EARLRRELTELGFRHRIVAAPNPAAARVLANIYDGLAVQDDGLMQALAPLPIDRAGLDPQ
AATALSRMGLRTLAQVQALPRHTLARRFDAGLLKHLDALVGHRPLGLAFYQPPDRFDVRI
ELNFDVQSHQALLFPLRRLTGDLSAFLCGRDSGVQRFDLHLEHAQAPDSVIKVGLLSAER
EPAMLFELARGRLEQVQVTSPVRGFRLVAQDLPVFVPQRQDLFDDRPQQTLPWEQLRERL
RARLGDEAVQGLRFHADHRPECAWQAAADKTLCPTLNNVQRPGWLLNEPTLLGEQGVQIL
MGPERIESGWWDGADIRRDYYLILTRAGQQGWAFRTVGQGDGLWLQGWFA