Protein Info for PS417_15580 in Pseudomonas simiae WCS417

Annotation: DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1023 TIGR00594: DNA polymerase III, alpha subunit" amino acids 4 to 968 (965 residues), 539.6 bits, see alignment E=8.8e-166 PF02811: PHP" amino acids 5 to 108 (104 residues), 61.7 bits, see alignment E=2.8e-20 PF07733: DNA_pol3_alpha" amino acids 268 to 522 (255 residues), 293.8 bits, see alignment E=3.4e-91 PF17657: DNA_pol3_finger" amino acids 525 to 691 (167 residues), 205.4 bits, see alignment E=9.2e-65 PF14579: HHH_6" amino acids 765 to 853 (89 residues), 66.2 bits, see alignment E=6.2e-22 PF01336: tRNA_anti-codon" amino acids 933 to 1004 (72 residues), 30.9 bits, see alignment 5.4e-11

Best Hits

Swiss-Prot: 83% identical to DNAE2_PSEPF: Error-prone DNA polymerase (dnaE2) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 94% identity to pfs:PFLU3602)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U2M5 at UniProt or InterPro

Protein Sequence (1023 amino acids)

>PS417_15580 DNA polymerase (Pseudomonas simiae WCS417)
MSYAELHCLSNFSFQRGASSALELFERAKRQGYSALAITDECTLSGIVRAWQAAKAVELP
LIIGSEVRIDNGPKLVLLVEDLSGYQHLCRLITLARRRAEKGSYRLLREDFDQPLPGLLA
LWVAEDSDTQADSQWLRRTFGERLWLAVHLHCGQNDARHLEQRLQLAASLHLPAVACGDV
HMHARGRRALQDTMTAIRHHVPVAEAGTRLHPNGERHLRSLDALAALYPQPLLDETETIA
RRCTFDLSQLRYHYPHELVPAGHNAQSWLRAVTEEGIAKRWPQGVDAKTAQQINSELELI
SELGYESYFLTVHDIVQFARSRSILCQGRGSAANSAVCYALGITEIDPSLTSMLFERFLS
RERNEPPDIDVDFEHERREEVLQYVFQRYGRTRAALTAVVSSYHASGAVRDVAKALGLPP
DQINALADCCGRWSDAAPPLDRLREGGFDPDSPILRRVLTLTQQLIGFPRHLSQHPGGFV
ISEYPLDTLVPVENAAMAERTIIQWDKDDLDAVGLLKVDILALGMLSAIRRCFDLLRRHR
NRDLALATIPKEDPATYAMISKADTIGVFQIESRAQMSMLPRLKPQKFYDLVIEVAIVRP
GPIQGGMVHPYLRRRNKEEETTYPSPELKVVLERTLGIPLFQEQVMQIAMVAADYGPGEA
DQLRRSMAAWKRHGGLEPHQERLRTGMLKNGYSEAFAAQIFEQIKGFGSYGFPESHAASF
ALLTYASCWLKCHEPAAFACALINSWPMGFYSPDQILQDARRHRLQIRPVDVQASDWDCS
LEPIDGAQPAIRMGLRMISGFREEDARRIEAARHRRAFSDIADLDDRAGLDARAQALLAD
AGALRALAGNRHKARWEVAGVHKQLGLFAGLPSPDEAVVELPSPTVGEDLHADYATVGTT
LGPHPLALLRAELRKRRCRSSQELMAVEHGRNVSVAGLVTGRQRPGTASGVTFVTLEDEF
GNLNVVVWRDLAERQRQALVGSRLMKVDGRWEAVGEVRHLIAGRLTDLTPLLEGITVRSR
DFH