Protein Info for GFF3034 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Sorbitol dehydrogenase (EC 1.1.1.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 PF08240: ADH_N" amino acids 25 to 130 (106 residues), 112.4 bits, see alignment E=1.4e-36 PF01262: AlaDh_PNT_C" amino acids 161 to 208 (48 residues), 27.4 bits, see alignment 3.2e-10 PF00107: ADH_zinc_N" amino acids 171 to 298 (128 residues), 83.1 bits, see alignment E=2.6e-27

Best Hits

Swiss-Prot: 55% identical to LGOD_ECOLI: L-galactonate-5-dehydrogenase (lgoD) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 99% identity to sei:SPC_3145)

MetaCyc: 55% identical to L-galactonate oxidoreductase (Escherichia coli K-12 substr. MG1655)
RXN0-5229 [EC: 1.1.1.414]

Predicted SEED Role

"Sorbitol dehydrogenase (EC 1.1.1.14)" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization (EC 1.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.14 or 1.1.1.414

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (338 amino acids)

>GFF3034 Sorbitol dehydrogenase (EC 1.1.1.14) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MKTLICNNPGNIEYIERDIPHLKDDEVLLKIKAVGICGTDIHAFAGRQPFFAYPRVLGHE
ICGVAEMLGKSCSTAKVGQRYSVIPCIPCGACAACREGKTNCCENVSLYGVHQDGGFSEY
LAVREQNLVELSDNLTDSAGALVECFAISAHAVRRADVKPQQNIVVVGAGPIGLAAAAIA
KAKGARVAVADIDAERRRLVAEKVGVATLDPSSDDYIDALKACFSGELAGTVLDATGNKS
SMSRAVDLIRHGGKIVFIGLYIGELVIDDPTFHKKETTLLSSRNATREDFECVIELMAQG
AISETMMKNQEFDFYTFGNQYQKNVVENKKLVKGVIKF