Protein Info for GFF3032 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Methylisocitrate lyase (EC 4.1.3.30)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 275 PF13714: PEP_mutase" amino acids 2 to 231 (230 residues), 154.3 bits, see alignment E=4.3e-49 PF00463: ICL" amino acids 61 to 150 (90 residues), 50 bits, see alignment E=1.8e-17

Best Hits

Swiss-Prot: 46% identical to CPPM_STRHY: Carboxyvinyl-carboxyphosphonate phosphorylmutase (bcpA) from Streptomyces hygroscopicus

KEGG orthology group: None (inferred from 70% identity to aaa:Acav_2476)

MetaCyc: 46% identical to carboxyphosphonoenolpyruvate phosphonomutase monomer (Streptomyces hygroscopicus)
Carboxyvinyl-carboxyphosphonate phosphorylmutase. [EC: 2.7.8.23]; 2.7.8.23 [EC: 2.7.8.23]

Predicted SEED Role

"Methylisocitrate lyase (EC 4.1.3.30)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.1.3.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.30

Use Curated BLAST to search for 2.7.8.23 or 4.1.3.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (275 amino acids)

>GFF3032 Methylisocitrate lyase (EC 4.1.3.30) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VIVPGAFNALSARIVEQLAFPAVYLTGAGLSNMSLGLPDLGFIGLAEVAEHTTRIRDAVD
LPLIVDADTGFGNALNVRHTVRVLERAGADAIQIEDQVAPKRCGHFVGKAVIAIDEAVGK
IQAAVDARRDADLMIVARTDAAASLGFDAAVERAQRFAEAGADLLFVEALTRSEDMQRLP
TLLDKPLLMNMVIGGKTPLVPAPQLAAWGYSFVLYANAALQGASRGMQRALLALRENGGL
NEDPALVTPFEERQRLVDKAGWDALEARYAGGVSR