Protein Info for GFF3031 in Xanthobacter sp. DMC5

Annotation: Methanol dehydrogenase [cytochrome c] subunit 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 628 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 504 to 520 (17 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 26 to 569 (544 residues), 666 bits, see alignment E=2e-204 PF01011: PQQ" amino acids 44 to 374 (331 residues), 141.4 bits, see alignment E=4.2e-45 amino acids 483 to 561 (79 residues), 48.5 bits, see alignment E=5.9e-17 PF13360: PQQ_2" amino acids 159 to 239 (81 residues), 31.9 bits, see alignment E=1.1e-11 amino acids 483 to 552 (70 residues), 19.9 bits, see alignment E=5.3e-08

Best Hits

Swiss-Prot: 82% identical to DHM1_METEA: Methanol dehydrogenase [cytochrome c] subunit 1 (moxF) from Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)

KEGG orthology group: K14028, methanol dehydrogenase (cytochrome c) subunit 1 [EC: 1.1.2.7] (inferred from 88% identity to sno:Snov_4188)

MetaCyc: 81% identical to methanol dehydrogenase large subunit (Methylobacterium organophilum)
1.1.2.7,1.1.2.e [EC: 1.1.2.7, 1.1.2.e]; 1.1.2.7 [EC: 1.1.2.7, 1.1.2.e]

Predicted SEED Role

"Methanol dehydrogenase large subunit protein (EC 1.1.99.8)" in subsystem Respiratory dehydrogenases 1 (EC 1.1.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.7, 1.1.99.8

Use Curated BLAST to search for 1.1.2.7 or 1.1.2.e or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (628 amino acids)

>GFF3031 Methanol dehydrogenase [cytochrome c] subunit 1 (Xanthobacter sp. DMC5)
MTRLFSSVSLCAVTLALAAGSAAVSSQALANDKLVELAKSDDNWPMTGKNYDANNYSPQK
QINKANVKQLRPAWSFSTGVLSGHEGTPLVVNNVMYIHSPYPNTTFAVGLDDPGHILWQH
KPKQNPTARAVACCDVVNRGLAYWPGDGKTPALILKTQLDGHIVALNAETGAEFWKLENS
DIKVGSTLTIAPYVVKDTVLVGSSGAELGVRGYVTAYDVKTGAQKWRAYATGPDEQVKLA
NDFNKANPQYGQKGLGTGTWEGDAWKIGGGTNWGWYAFDPGTNMIYYGSGNPAPWNETMR
PGDNKWTMTIWGRDIDTGEAHFGYQKTPHDEWDYAGVNVMMLSEQKDKAGKMRKLLTHPD
RNGIVYTLDRTDGSLVSADKIDDTVNWVKQVDLKTGLPQRDPEYATRMDHKGRDICPSAM
GYHNQGHDSYDPERQSFFMGINHICMDWEPFMLPYRAGQFFVGATLWMYPGPKGNKQTYE
GLGQVKAYDAINNKYKWEVMERFAAWGGTLATAGGVMFYGTLDGFIKARDSDTGELLWKF
KLPSGVIGHPMTYTHKGVQYVAIYYGVGGWPGVGLVFDLNDPTAGLGSVGAFKQLQNYTQ
MGGGVMVFSLDGKGPYDDPKVGEYASGG