Protein Info for GFF3027 in Sphingobium sp. HT1-2

Annotation: HoxN/HupN/NixA family nickel/cobalt transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 transmembrane" amino acids 15 to 36 (22 residues), see Phobius details amino acids 81 to 106 (26 residues), see Phobius details amino acids 119 to 141 (23 residues), see Phobius details amino acids 192 to 215 (24 residues), see Phobius details amino acids 221 to 243 (23 residues), see Phobius details amino acids 263 to 287 (25 residues), see Phobius details amino acids 313 to 332 (20 residues), see Phobius details PF03824: NicO" amino acids 43 to 330 (288 residues), 312.2 bits, see alignment E=1.8e-97 TIGR00802: transition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family" amino acids 46 to 325 (280 residues), 381.5 bits, see alignment E=1.3e-118

Best Hits

Swiss-Prot: 56% identical to HOXN_CUPNH: High-affinity nickel transport protein (hoxN) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K07241, high-affinity nickel-transport protein (inferred from 70% identity to npp:PP1Y_AT19766)

Predicted SEED Role

"HoxN/HupN/NixA family cobalt transporter" in subsystem Coenzyme B12 biosynthesis or Transport of Nickel and Cobalt

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (350 amino acids)

>GFF3027 HoxN/HupN/NixA family nickel/cobalt transporter (Sphingobium sp. HT1-2)
MTAPPSQASLRRRSVPLIAALVIANLAVWGWAFALFGDRPVMMGTALLAWGLGLRHAVDA
DHIAAIDNVTRKLMQDGQRPLTVGLWFAIGHSAIVLIAALGLAYAVSALAGLERFKATGG
IVATLISATFLFAIAIMNLVILRQVWTTFRRVQAGGTYVEADLDMLTGGRGPLSRLFRPV
FRLISRPWQMAPLGLLFGLGFDTATEVAILGLSAAQASDGFALGPVLVLPLLFAAGMALV
DTVDGMLMMGAYQWAFVKPLRKLYYNIVITALSAAVAIGIGTIETLALLGDKLGLSGGIW
DVAATLGEHFNDLGFAIIGLFIACWIASALFYRWKGYDAIELTPAGEVRA