Protein Info for GFF3021 in Sphingobium sp. HT1-2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1067 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF07676: PD40" amino acids 75 to 110 (36 residues), 34.4 bits, see alignment (E = 4.8e-12) amino acids 131 to 148 (18 residues), 14.5 bits, see alignment (E = 8.6e-06) amino acids 230 to 258 (29 residues), 14.3 bits, see alignment (E = 9.6e-06) amino acids 393 to 409 (17 residues), 22 bits, see alignment (E = 3.8e-08) PF01979: Amidohydro_1" amino acids 974 to 1037 (64 residues), 45.5 bits, see alignment 2e-15

Best Hits

Predicted SEED Role

"tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1067 amino acids)

>GFF3021 hypothetical protein (Sphingobium sp. HT1-2)
MRSLASLLLGASLLCAHPALAQDSKGLSLTPTRTLRYDAHEATWMQPDMSPDGRTILFDL
LGDIYALDAGGGTARPLLTGMAFERNPVFSPDGKQFAFLSDRSGVTNLWIANVDGTGLKQ
LSQDQSLAIYSSPAWSPDGRSVYVSRTVHSILAFELFMYDRDGGSGIQITKAGSPDNWDA
KMNAMGAVATPDGRYLYYATKRGHTWTENDLPNWSIARRDLKSGQDETIIQSAGGAMRPA
LSHDGKLLAYASRQGQKDGLRLRNLETGEDRWIAFPIDRDGQDGGYYADLLPRFSFLPGD
KAMLMSVGGGIRRLDIASGKISDIPFTAPVQLGLGPLTRVRQTEETGPVRVRVVQAPRQS
PDGGSVAFTALGGLYVQKLTANMTPKRIVAADAFQPSWSPDGKSIAYVSWTAKDGGHVWT
IPATGGTPRRLTQIPAYYSQPIFTPDGKGVVALRANQYDRLRTASEIDPARPTDIIHMPA
SGGAARLVAHAMGARQPQFGKDRDTLYFYGAGGLSSVPLAGGDPQPLLRVLAHGLGQYFP
GPAPVEDLRISPDGSRALIKSFSQAYLVDIPPANGGEPATVNLDTPAVARRKLTRVGADF
IDWADKGATVTWSVGSTFRRLPTDQALSAAPGTAETAAQSFALPVELPRDVPRGSVVLRG
ATVMPMAGGQDVIRNADIVITDNRIVAVGASGSVAVPQGATIRDVGGKYIVPGFVDTHAH
WFEIRRGIQERGHWDFAANLAFGVTSALDPQPFTTDIFAYQDMIDAGMMRGPRAWSTGPG
VFVNAEIHSKQDAVDVLTRYRDHYRTRNIKSYMIGDRAQRQYMVEAAAELGMMPTTEGAS
DMVLNLTHAIDGFSGNEHSIPVSPLREDIVRLFAQSRTSYTPTILVAYGTTPALFDFIIN
KRPQDDAKWTHFVPAGIVSDKLRNRHWMPPEAQSWQQIAADAVQIQRAGGLVGLGSHGEI
QGLGYQWELQAFAAGGATPIEALKAATIGSAEVIGHSDDVGSLEPGKFADLLILDADPLA
DIANAQKIGAVMKNGRIYDPATLDEIWPTPAKLAEPWFAQDGATPTP