Protein Info for GFF302 in Variovorax sp. SCN45

Annotation: Phosphoribosylamine--glycine ligase (EC 6.3.4.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02844: GARS_N
  • amino acids 1 to 100 (100 residues), 129.2 bits, E=1.3e-41
• TIGR00877: phosphoribosylamine--glycine ligase
  • amino acids 1 to 424 (424 residues), 560.5 bits, E=1.1e-172
• PF01071: GARS_A
  • amino acids 101 to 298 (198 residues), 259.6 bits, E=2.9e-81
• PF02843: GARS_C
  • amino acids 333 to 422 (90 residues), 105.3 bits, E=2.6e-34

Best Hits

Swiss-Prot: 72% identical to PUR2_RALSO: Phosphoribosylamine--glycine ligase (purD) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 96% identity to vap:Vapar_2684)

MetaCyc: 60% identical to phosphoribosylamine--glycine ligase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylamine--glycine ligase. [EC: 6.3.4.13]

Predicted SEED Role

"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.13

Use Curated BLAST to search for 6.3.4.13

Protein Sequence (433 amino acids)

>GFF302 Phosphoribosylamine--glycine ligase (EC 6.3.4.13) (Variovorax sp. SCN45)
MKVLVIGGGGREHALAWRLAQATRVSRVYVAPGNGGTASDERYDCIDITEPAALREWAIK
EKIALTVVGPEAPLAAGVVDEFREHGLRIFGPTQAAAQLESSKAFSKAFMKRHKIPTAEY
EAFTDAALAHAYIDAKGAPIVVKADGLAAGKGVVVAMTAQEAHEAVDFMLVDNKFGVSHN
EGGARVVIEEFLQGEEASFIVLCDGKNVTALATSQDHKRLLDNDEGPNTGGMGAYSPAPV
VTAEVHARAMREIILPTIRGMEKDGIPYTGFLYAGLMIDAAGHPKTLEFNCRMGDPETQP
IMMRLKSDLFEVFWHATDGTLDQVELQWDRRVALGVVMAAHGYPLSPRKGDRITGIPAEA
PDAVVFHAGTTLDNGELKTSGGRVLCVTVLADSVKLAQQRVYEVAARVKFDGAQYRKDIG
FRALHARNTPPNA