Protein Info for GFF302 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Glutamyl-tRNA synthetase (EC 6.1.1.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00749: tRNA-synt_1c
  • amino acids 5 to 311 (307 residues), 310.5 bits, E=1.1e-96
• TIGR00464: glutamate--tRNA ligase
  • amino acids 5 to 460 (456 residues), 531.4 bits, E=1.1e-163
• PF19269: Anticodon_2
  • amino acids 330 to 461 (132 residues), 90.3 bits, E=1.6e-29

Best Hits

Swiss-Prot: 84% identical to SYE_RHOFT: Glutamate--tRNA ligase (gltX) from Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 84% identity to rfr:Rfer_2681)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• superpathway of heme b biosynthesis from glutamate (9/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.17

Protein Sequence (462 amino acids)

>GFF302 Glutamyl-tRNA synthetase (EC 6.1.1.17) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSAPIRTRFAPSPTGFIHLGNIRSALYPWAFARSTGGAFILRIEDTDLERSSQAAVDVII
EGMKWLGLDHDEGPFYQMQRMDRYKAVLAEMVAGGLVYPCYMSVAELDALREKQMAAKEK
PRYDGTWRPEPGKTLPPVPEGVKPVLRFKNPQGGVVAWDDKVKGRIEIRNDELDDLVIAR
PDGTPTYNFCVVVDDIDMAISHVIRGDDHVNNTPRQINIFRALGKEPPVYAHLPTVLNEQ
GEKMSKRNGAKPVTQYRDEGYLPDAMVNYLARLGWSHGDDEIFSRAQFLEWFNLDHLGRS
AAQFDEAKLKWVNAQHLKAMADDALAPLVAAHLQQQGIEADARLPRICGLFKDRCDTTVA
LAGWAKAFYADITPNAEERAQHVTDAVRPVLVTLADKLAACAWDKAALSAAIKEVLAAHG
LKMPQLAMPVRVLVMGTAQTPSLDAVLELHDREVVLRRLQNG