Protein Info for GFF3012 in Sphingobium sp. HT1-2

Annotation: Amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF01979: Amidohydro_1" amino acids 76 to 417 (342 residues), 126.8 bits, see alignment E=1.2e-40

Best Hits

KEGG orthology group: None (inferred from 73% identity to nar:Saro_2515)

Predicted SEED Role

"amidohydrolase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (425 amino acids)

>GFF3012 Amidohydrolase (Sphingobium sp. HT1-2)
MHRLLILLAFLFVAPMPAGAEQAAPALYLLRPDAVFDGATSHRGWAVLVRGNRIEAAGPG
IAVPAGARVIGLPGKTLLPGLIEGHSHLFLHPYNETSWDDQVLHEPLALRTVRATVSARA
TLMAGFTTVRDLGTEGAGYADVGLKQAIEQGIVAGPRMLVATRALVAPGAYGPRGFEPGV
VVPLGAEEAGGPDLMTAVRRQIAAGADVVKLYADYRWGKGEPSRPTFTQAEMAAAVEAAH
SAGRKVAAHAATDEGMRRAILAGVDTIEHGYEGSAATFALMKAHNVGYCPTLAASDATAR
YRGWTGAEPAPAAVTQARQALDRARAAGVAICLGGDVGVFAHGDNAREAELMAAAGMAPR
DVLVAATSGNAALFGIADRLGAVKPGLLADLVAVEGDPTRSIGALRQVGFVMKDGVIYKG
PGAQP