Protein Info for GFF30 in Xanthobacter sp. DMC5

Annotation: Sialic acid TRAP transporter permease protein SiaT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 623 transmembrane" amino acids 30 to 53 (24 residues), see Phobius details amino acids 65 to 82 (18 residues), see Phobius details amino acids 103 to 125 (23 residues), see Phobius details amino acids 145 to 166 (22 residues), see Phobius details amino acids 173 to 191 (19 residues), see Phobius details amino acids 203 to 235 (33 residues), see Phobius details amino acids 255 to 283 (29 residues), see Phobius details amino acids 294 to 317 (24 residues), see Phobius details amino acids 337 to 360 (24 residues), see Phobius details amino acids 367 to 392 (26 residues), see Phobius details amino acids 413 to 431 (19 residues), see Phobius details amino acids 438 to 457 (20 residues), see Phobius details amino acids 469 to 493 (25 residues), see Phobius details amino acids 512 to 546 (35 residues), see Phobius details amino acids 557 to 582 (26 residues), see Phobius details amino acids 597 to 619 (23 residues), see Phobius details PF04290: DctQ" amino acids 42 to 168 (127 residues), 85 bits, see alignment E=4.5e-28 PF06808: DctM" amino acids 210 to 617 (408 residues), 337.9 bits, see alignment E=8.7e-105 TIGR00786: TRAP transporter, DctM subunit" amino acids 218 to 619 (402 residues), 279 bits, see alignment E=2.9e-87

Best Hits

KEGG orthology group: None (inferred from 72% identity to bbt:BBta_2025)

Predicted SEED Role

"Predicted gluconate TRAP family transporter, DctM subunit" in subsystem D-gluconate and ketogluconates metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (623 amino acids)

>GFF30 Sialic acid TRAP transporter permease protein SiaT (Xanthobacter sp. DMC5)
VQLEMGIAPARAVAATGIPARIGTLIRNVTGAAVAVLVVAEILVLFAGVLARYVFHAPLI
WSDELASILFLWLAMLGAVVAFERGEHMRMSAFVGGLRQPFRGFFDALAIAVPLLFLSLA
LGAAYEFAVDEIIVSTPALDISAGWRVAALPVGFALMLVLAVLRLIERTDWRVTLAAVVL
VVVAAAVFTALRPSFTGLGNLNLLIFFVGLVGAMVLAGIPIAFAFATATFGYIALTTRLP
HSVVIGRMDEGMSHFVLLSVPLFVFLGVLIEMTGMARAMLAFLASLLGHVRGGLQYVLVG
AMYLVSGISGSKAADMAAIAPVLLPEMEKRGADRGELLALLAATGAQTETIPPSLVLITI
GSVTGVSIAALFAGGLMPALVVGALLCCVVWLRSRKDDLSGVRRASFGEIAKALVVALPA
IALPFIIRAAVIEGVATATEVSTIGIAYAVLAGLLIYRRFDWRRLYPMLVDTAALSGAIL
LIIGAATGMAWALTQSGFSAALAHMMTSLPGGAVGFMLVSLVAFIVLGSLLEGIPAIVLF
GPLLFPIARQVGVNEVHYSMVVILAMGIGLFAPPFGVGYYAASAISRIRPDEGMRPMIGY
IAALFIGTLIVAAVPWISTGFIR