Protein Info for GFF2995 in Xanthobacter sp. DMC5

Annotation: Hemin import ATP-binding protein HmuV

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 251 PF00005: ABC_tran" amino acids 19 to 160 (142 residues), 102.7 bits, see alignment E=1.3e-33

Best Hits

Swiss-Prot: 44% identical to HMUV_PSEE4: Hemin import ATP-binding protein HmuV (hmuV) from Pseudomonas entomophila (strain L48)

KEGG orthology group: K02013, iron complex transport system ATP-binding protein [EC: 3.6.3.34] (inferred from 56% identity to mno:Mnod_8245)

MetaCyc: 37% identical to iron(III) hydroxamate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-11-RXN [EC: 7.2.2.16]; TRANS-RXN-297 [EC: 7.2.2.16]; TRANS-RXN-298 [EC: 7.2.2.16]

Predicted SEED Role

"Vitamin B12 ABC transporter, ATPase component BtuD" in subsystem Coenzyme B12 biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.34

Use Curated BLAST to search for 3.6.3.34 or 7.2.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (251 amino acids)

>GFF2995 Hemin import ATP-binding protein HmuV (Xanthobacter sp. DMC5)
MNLAARSVTVTRGAATLVRDLSLAVEGGTFLGLLGPNGAGKTSLMRVLAGLEAPASGHVL
YDGRDAAEIGRRGLGRTIAYLPQDGRIHWPLPVEEVVALGRLPHGTAAVDEAARRAMAAA
GVEALARRSADTLSGGERARVLLARALAVEAPILLADEPSAALDPLHQLEVMALLRDTAQ
RGAVVVAVLHDLTLAGRFCDRLALMKDGRLLAEGTAAQVLDDAHLAEAFGVDAVRLTHAG
EMVVLPWSRRG