Protein Info for GFF2987 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 505 TIGR00499: lysine--tRNA ligase" amino acids 14 to 504 (491 residues), 790.3 bits, see alignment E=3.3e-242 PF01336: tRNA_anti-codon" amino acids 68 to 146 (79 residues), 63.2 bits, see alignment E=2.7e-21 PF00152: tRNA-synt_2" amino acids 162 to 501 (340 residues), 322.5 bits, see alignment E=4.4e-100

Best Hits

Swiss-Prot: 100% identical to SYK_SALPA: Lysine--tRNA ligase (lysS) from Salmonella paratyphi A (strain ATCC 9150 / SARB42)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 99% identity to ses:SARI_04610)

MetaCyc: 95% identical to lysine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Lysine--tRNA ligase. [EC: 6.1.1.6]

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (505 amino acids)

>GFF2987 Lysyl-tRNA synthetase (class II) (EC 6.1.1.6) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSEQNAQGADEVVDLNNEMKARREKLAALREQGIPFPNDFRRDRTSDQLHAEFDAKEAEE
LEALNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGD
ILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGLQDQEARYRQRYLDLISNDES
RNTFKTRSKILAGIRQFMVARGFMEVETPMMQVIPGGASARPFITHHNALDLDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRT
LAQDVLGTTQVPYGDEVFDFGKPFEKLTMREAIKKYRPETDMADLDNFDSAKAIAESIGI
HVEKSWGLGRIVTEIFDEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGG
REIGNGFSELNDAEDQAQRFLDQVNAKAAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPVK