Protein Info for GFF2986 in Sphingobium sp. HT1-2

Annotation: Osmosensitive K+ channel histidine kinase KdpD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 886 transmembrane" amino acids 394 to 413 (20 residues), see Phobius details amino acids 422 to 451 (30 residues), see Phobius details amino acids 471 to 489 (19 residues), see Phobius details PF02702: KdpD" amino acids 21 to 228 (208 residues), 320.7 bits, see alignment E=9.3e-100 PF13493: DUF4118" amino acids 395 to 499 (105 residues), 83.2 bits, see alignment E=2.7e-27 PF13492: GAF_3" amino acids 520 to 639 (120 residues), 38.6 bits, see alignment E=3.5e-13 PF00512: HisKA" amino acids 659 to 719 (61 residues), 51.3 bits, see alignment 2.5e-17 PF02518: HATPase_c" amino acids 764 to 873 (110 residues), 76.6 bits, see alignment E=5e-25

Best Hits

KEGG orthology group: K07646, two-component system, OmpR family, sensor histidine kinase KdpD [EC: 2.7.13.3] (inferred from 63% identity to sch:Sphch_1027)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (886 amino acids)

>GFF2986 Osmosensitive K+ channel histidine kinase KdpD (Sphingobium sp. HT1-2)
VNEQHRPDPEAFLRAAAQEGRGRLKVFLGAAPGVGKTWEMLTEGRQRREAGVDVVVGVVE
THGRRETEALVHGHEIIPRRTVDHQGHSLGEMDIDAILARHPQLALVDELAHSNAPGSRH
PKRYQDVEELLAAGIDVYSTINIQHVESLNDVVASFTRVRVRETVPDSILEHAEIEVVDI
PPDELIERLKDGKVYIPQEASRALNHFFSKSNLTALRELALRRAAQAVDAQMLDYVRAHA
LAGSFAAGERVIVAVSEQPSAAGLVRSAKRLADALRAPWTAIHIETPRGPQLSDEDRQRL
ADTLALASRLGAETASIPAPSVADGLRGFAADARATQIVIGKSARSWWFELRHGSVVDRL
VRDIGPVAVHVMPGEESAPRPRAASTGHWGDPIGYVWSMLAVIGMTLLGRLMLEVIDLGN
IALLYLVPVMFAAASHGLRTGLATGLASSLAYNFFFLPPTGTLTVSNPENVISILVFLGV
AVVTSQFAARMHAQSVLAQSSARQNATLAGFSRQLTASPSLEEIRHAICAEIARLFDVRT
VLMIPSDEGPQLRAAYPPEDRLDEIERAAARWAMDHGEPAGRGSSTLTASDWLFLPVTAK
DAVLAVIGLAREDAGEPVRSDQFPLLMSLIDQAAIALDRMALEEAMLEAGQIRERDRLRS
ALLSSVSHDLRTPLTTIISATRELRTHPSPELIDTLDGEAQRLSRFVANLLDMARVEAGA
LPMRAEATDLFDAVASAAHDSRSALADRQLDVQIPPDIPLVRVDPVLLHHILINLLDNAG
RYGDPGTPVIVRSLREPDAISLSVIDQGPGIPAGMEARVFDTFTRLEGSDRSKTGTGLGL
AIVKAFAEAMGLSVSARTVADPPGACFTLHIPQSLILTDIMEKDIA