Protein Info for GFF2984 in Sphingobium sp. HT1-2

Annotation: Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 682 transmembrane" amino acids 38 to 58 (21 residues), see Phobius details amino acids 70 to 88 (19 residues), see Phobius details amino acids 220 to 243 (24 residues), see Phobius details amino acids 249 to 276 (28 residues), see Phobius details amino acids 587 to 610 (24 residues), see Phobius details amino acids 616 to 636 (21 residues), see Phobius details amino acids 654 to 675 (22 residues), see Phobius details TIGR01497: K+-transporting ATPase, B subunit" amino acids 8 to 676 (669 residues), 983.8 bits, see alignment E=3.7e-300 TIGR01494: HAD ATPase, P-type, family IC" amino acids 74 to 343 (270 residues), 107.2 bits, see alignment E=7.4e-35 amino acids 368 to 599 (232 residues), 152 bits, see alignment E=1.9e-48 PF00122: E1-E2_ATPase" amino acids 107 to 284 (178 residues), 102.9 bits, see alignment E=1.5e-33 PF00702: Hydrolase" amino acids 301 to 530 (230 residues), 84.5 bits, see alignment E=1.3e-27

Best Hits

Swiss-Prot: 70% identical to KDPB_AGRFC: Potassium-transporting ATPase ATP-binding subunit (kdpB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01547, K+-transporting ATPase ATPase B chain [EC: 3.6.3.12] (inferred from 86% identity to sch:Sphch_1025)

MetaCyc: 64% identical to K+ transporting P-type ATPase subunit KdpB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-2 [EC: 7.2.2.6]

Predicted SEED Role

"Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)" in subsystem Potassium homeostasis (EC 3.6.3.12, TC 3.A.3.7.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.12

Use Curated BLAST to search for 3.6.3.12 or 7.2.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (682 amino acids)

>GFF2984 Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1) (Sphingobium sp. HT1-2)
MARSSNKSEQKSLFTADLIVPACRDAFRKLNPQELIRNPVMFTTAIVAVLLTVLLLVGQD
SLAVGFKLQLVIWLWLTVLFGTFAEALAEGRGKAQAASLRATKADLTAKRLKGHGESYEN
VAASALKLGDVVLVQTGDLIPSDGEVVSGVASVNEAAITGESAPVIREAGGDRSAVTAGT
RVISDEIRVRVTVNPGQGFLDRMIALVEGAERQKTPNEIALTLLLVGLTIIFLIAVGTIP
SFASYAGGAIPVAILAALLITLIPTTIAALLSAIGIAGMDRLVRFNVLAKSGRAVEAAGD
IDVLLLDKTGTITVGDRQATEFRPVGGATPVQLAEAALLASLADETPEGRSIVLLAREGF
NQSVDALPANAEIIPFTAQTRISGVTIDGSTIQKGAVDSVLKANHGLGSTPVANELRRIT
DEIARAGGTPLAVAKDGKLLGAIFLKDIVKAGIRERFGELRQMGIRTVMITGDNPLTAAS
IAAEAGVDDFLAQATPEDKLALIRKEQQGGRLVAMCGDGTNDAPALAQADVGVAMNTGTQ
AAREAGNMVDLDSDPTKLIEVVGLGKQLLMTRGALTTFSVANDVAKYFAIIPAMFVALYP
GLGILNVMGLATPESAILSAIIFNALIIPCLVPLALKGVTYRPIGAGPLLARNLAIYGLG
GLIAPFVGIKLIDLAVNGLGLA