Protein Info for GFF2956 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868
Annotation: Glutamate synthase [NADPH] large chain (EC 1.4.1.13)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to GLTB_ECOLI: Glutamate synthase [NADPH] large chain (gltB) from Escherichia coli (strain K12)
KEGG orthology group: K00265, glutamate synthase (NADPH/NADH) large chain [EC: 1.4.1.13 1.4.1.14] (inferred from 61% identity to abo:ABO_2229)MetaCyc: 96% identical to glutamate synthase subunit GltB (Escherichia coli K-12 substr. MG1655)
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- superpathway of ammonia assimilation (plants) (2/3 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (1/4 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1486 amino acids)
>GFF2956 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868) MLYDKSLEKDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGAILADGKTGDGCGLLLQ KPDRFFRIVAEERGWRLAKNYAVGMIFLNKDPELAAASRHIVEEELQQETLSIVGWRDVP TNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIARRRIEKRLQDDKDFYVCSLS NLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRFSTNTVPRWPLAQPFRYLAHNGE INTITGNRQWARARTYKFQTPLIPDLQSAAPFVNETGSDSSSLDNMLELLLAGGMDIIRA MRLLVPPAWQNNPDMDQDLRAFFDFNSMHMEPWDGPAGIVMSDGRFAACNLDRNGLRPAR YVITKDKLITCASEVGIWDYQPDEVVEKGRVGPGELMVIDTRGGRILHSAETDDDLKSRH PYKEWMEKNVRRLVPFEELPDEEVGSRELDDDLLASYQKQFNYSAEELDSVIRVLGENGQ EAVGSMGDDTPFAVLSSQPRIIYDYFRQQFAQVTNPPIDPLREAHVMSLATSIGREMNVF CEAEGQAHRLSFKSPILLYSDFKQLTTMKEEHYRADRLDITFDVTETTLNATVKALCDKA EQMVRNGTVLLVLSDRNIGKNRLPVPAPMAVGAVQTRLVEQSLRCDANIIVETGSARDPH HFAVLLGFGATAIYPYLAYETLGRLIDTQAIAKNYRTVMQNYRNGINKGLYKIMSKMGIS TIASYRCSKLFEAVGLHDDVVNLCFQGVVSRIGGASFDDFQQDLLNLSKRAWLARKPISP GGLLKYVHGGEYHAYNPDVVRTLQQAVQSGEYSDYQEYAKLVNERPASTLRDLLAIHPDG EAVTIDEVEPASELFKRFDTAAMSIGALSPEAHEALAEAMNSLGGNSNSGEGGEDPARYG TNKVSRIKQVASGRFGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSV PGVTLISPPPHHDIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADL ITIAGYDGGTGASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDII KAAILGAESFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYF EFIAREVRELMASLGVTRLVDLIGRTDLLKELEGFTAKQQKLALSRLLETAEPHPGKALY CTENNPPFDNGVLNAQLLQQAKPFVDARQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQG LASDPIKAHFSGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFLSH KASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCVLGK TGVNFGAGMTGGFAYVLDEDGEFRKRVNPELVEVLDVDSLAIHEEHLRGLITEHVQHTGS QRGEEILSRWSSFSTQFALVKPKSSDVKALLGHRSRSAAELRVQAQ