Protein Info for GFF2921 in Variovorax sp. SCN45

Annotation: D-galactarate dehydratase (EC 4.2.1.42)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 526 TIGR03248: galactarate dehydratase" amino acids 11 to 526 (516 residues), 933.8 bits, see alignment E=1e-285 PF08666: SAF" amino acids 19 to 85 (67 residues), 30 bits, see alignment E=9.2e-11 PF04295: GD_AH_second" amino acids 119 to 272 (154 residues), 115.7 bits, see alignment E=2.5e-37 PF20629: GD_AH_C" amino acids 282 to 522 (241 residues), 306.5 bits, see alignment E=1.9e-95

Best Hits

KEGG orthology group: K01708, galactarate dehydratase [EC: 4.2.1.42] (inferred from 93% identity to vpe:Varpa_1892)

Predicted SEED Role

"D-galactarate dehydratase (EC 4.2.1.42)" in subsystem D-Galacturonate and D-Glucuronate Utilization or D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.42)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.42

Use Curated BLAST to search for 4.2.1.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (526 amino acids)

>GFF2921 D-galactarate dehydratase (EC 4.2.1.42) (Variovorax sp. SCN45)
MSNAAAGASPLYIRIHANDNVAIVANDGGLKAGTEFADGLRLVDNVPQGHKVALSDLAEG
AAIVRYNVVIGYAQKALPRGSWVHERVMRMPVAPELDGLPIATVKAPALPPLEGYTFEGY
RNADGSVGSRNILAITETVQCVAGVIDFAVQRIKAELLPRYPNVDDVVALAHGYGCGVAI
DAPDAIVPIRTLRNISLNPNFGGEVMVVSLGCEKLQPERLLPPGSIPIADKRGEAEGDAE
LDVVCLQDDAHVGFMSMIDSVMRQAEQHLVRLNARKRETVPASELVVGVQCGGSDAFSGV
TANPAVGFCTDLLVRAGATVMFSEVTEVRDGIDQLTSRASSPEVAEAMIREMAWYDAYLQ
KGRVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGSAPITGVLAPGEKVAQKGLIYAATPA
SDFICGTLQLAAGINLHVFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAGRIAD
GEASIEDVGWELFRLMLDVASGRKKTWAEHWKLHNALVLFNPAPVT