Protein Info for GFF2895 in Xanthobacter sp. DMC5

Annotation: Caffeine dehydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 785 PF01315: Ald_Xan_dh_C" amino acids 25 to 133 (109 residues), 58.1 bits, see alignment E=1.5e-19 PF02738: MoCoBD_1" amino acids 153 to 395 (243 residues), 204.2 bits, see alignment E=2.7e-64 PF20256: MoCoBD_2" amino acids 424 to 712 (289 residues), 252.2 bits, see alignment E=1.1e-78

Best Hits

KEGG orthology group: K03520, carbon-monoxide dehydrogenase large subunit [EC: 1.2.99.2] (inferred from 92% identity to xau:Xaut_0648)

Predicted SEED Role

"Carbon monoxide dehydrogenase large chain (EC 1.2.99.2) parolog without usual motifs" in subsystem CO Dehydrogenase (EC 1.2.99.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.99.2

Use Curated BLAST to search for 1.2.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (785 amino acids)

>GFF2895 Caffeine dehydrogenase subunit alpha (Xanthobacter sp. DMC5)
MSEPRSHEGVGARLPRKEDDRLMRGRGQFIADIRMAGMQDVAFVRSPLAHARIRAIHVPD
DLKGRVFTAADLDGVKPIRAVSGLPGFKVSEQPVLATGKVRQVGELIAMCVADTRAEAED
MAARVELDLEELPALHDMLDARKPGAALVHEHWGDNVFLESLVNINIEAAFDAPVKISRT
ISTARQCMAPIEGRGTVVHFDHRSDQLVVHTASQMPHIVRSGLSECLGLEEGQIRIISPD
VGGGFGYKAILLSEDVCLGWLTRKIGRPVRWLEDRREHLTASANCREHHYEITLYAERDG
TLRGIDCEASVDSGAYSAYPFSACLEAAQVASILPGPYDFPAYRCRTWSVATNKCPILPY
RGVARTGVCFALEVALDALAAELGLAPEVVRLKNLVRPEQMPFDNITNKHFDSGNYPEAL
RQALDAIKADAVRTRQLAGEPDGRRIGLGLSIYCEQAAHGTSVYSGWGIPMVPGHEQAGA
RLTPDGGLELRIGAHSHGQGLETTLAQVAHEILGMPVARTRLVHGDTAMTPYSTGSWGSR
VMVMAGGAVATACTELSERIRRIGAHMLQARPEDVRLEAGRVIGPSGDVTIQQVAHTWYR
RPQDLPADVDPHGLEVTAGYKPVRDSGTFSYAAHAVVVAVDPDLGEVEILDYAIVEDGGT
LVNPMVVDGQIIGGLAQGIGTALYEEMAFDASGQPLASTLADYLLPGAPEVPMPFIGHME
TPSPYTKFGVKGIGEGGAIAPPAAIANAINDALRPLGAEMLRSPVTPRRIVEQVLAVRAG
ERSAA