Protein Info for PS417_14800 in Pseudomonas simiae WCS417

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 860 transmembrane" amino acids 25 to 46 (22 residues), see Phobius details amino acids 261 to 283 (23 residues), see Phobius details PF05228: CHASE4" amino acids 70 to 223 (154 residues), 75.7 bits, see alignment E=8.6e-25 TIGR00229: PAS domain S-box protein" amino acids 310 to 426 (117 residues), 33.3 bits, see alignment E=4.8e-12 PF13188: PAS_8" amino acids 313 to 359 (47 residues), 20.4 bits, see alignment 7.4e-08 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 428 to 590 (163 residues), 150.1 bits, see alignment E=4.6e-48 PF00990: GGDEF" amino acids 432 to 587 (156 residues), 161.4 bits, see alignment E=3.2e-51 PF00563: EAL" amino acids 609 to 839 (231 residues), 238.2 bits, see alignment E=1.7e-74

Best Hits

KEGG orthology group: None (inferred from 90% identity to pfs:PFLU3444)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TZW2 at UniProt or InterPro

Protein Sequence (860 amino acids)

>PS417_14800 diguanylate cyclase (Pseudomonas simiae WCS417)
MPNSLAPIVRSLSDRTSLSLARTSLFRFMALLAVVFAVAAYSLIFLAQELDRSEQVESAF
YTRRAVQSLEKSLRVTVKDYAFWSDAYRHLHVKVDDDWAFVRGNIGASLFDDFGFQGLFV
VDDANRTVYAVVDGQLQTVELTQWLDGPITGIIEQARAGADSETSATTFINVRGSPALVA
AAAITPGTDPTVLPDGRPASVLIFVDILDSAKLTAIGDEFGVDKLHIATPDEIGKTLLLP
LGDNGAAGSLHWEPARPGLRLMSVGLPLLGLAALLVCLMSWVISRRTTAAALALDASHSS
LQNSQNALATSEARFRDVVEASSDWVWEIDGDWRFTYLSERFEGVTGLVRDAWIGATIND
LLETETGLLSQWLSAPGRRPDISVQCRYVDALGLERSTRLSARQMPCGGFRGTATDVTEE
VEARRRIEFLSQHDALTGLPNRTRLQAFLEGKFKAMPTSEQPLVMLSLDLDRFKPVNDLL
GHAAGDRVLNEVSSRLADCVRHGDLVARIGGDEFVLILTDAGTQDEVEILCKRLIESIER
TIRIDEQEVFVSASIGIAIAPNDAREAAELLRYADIALYEAKAAGRNTWRFYSGDMNARI
IERRRLESDLRYGIKHGELRLYFQPRYRIADGQMVGAEALVRWQHPVRGLIAPDTFIPIA
EESGLILALSDWVLETACACAAQWPEHLFVSVNLSPTEFKRGNLVERTRQALAVSGIDPA
RVELEITESVMLEDASGALEVMHTLKRLGLRIAMDDFGTGYSSLSYLRAFPFDGLKIDRS
FLTRLEESDDDKAIIHAIVGLGRALALTVTAEGIETAEHLAMLKSVACEEGQGYFLSRPL
DFDGFNGLLGLHANVTQGAT