Protein Info for PS417_14735 in Pseudomonas simiae WCS417

Updated annotation (from data): Nitrate reductase (EC 1.7.99.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.99.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1324 PF04879: Molybdop_Fe4S4" amino acids 5 to 55 (51 residues), 63.7 bits, see alignment (E = 3.7e-21) PF00384: Molybdopterin" amino acids 80 to 488 (409 residues), 209 bits, see alignment E=3.3e-65 PF01568: Molydop_binding" amino acids 585 to 696 (112 residues), 122.2 bits, see alignment E=3.2e-39 PF00258: Flavodoxin_1" amino acids 809 to 936 (128 residues), 98.4 bits, see alignment E=1.3e-31 PF00667: FAD_binding_1" amino acids 959 to 1148 (190 residues), 107.1 bits, see alignment E=3.1e-34 PF00175: NAD_binding_1" amino acids 1182 to 1288 (107 residues), 59.7 bits, see alignment E=1.2e-19

Best Hits

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UAA3 at UniProt or InterPro

Protein Sequence (1324 amino acids)

>PS417_14735 Nitrate reductase (EC 1.7.99.4) (Pseudomonas simiae WCS417)
MANQSIRSVCPYCGVGCGIVMQVENNKVVKVIGDTAHPTNFGRLCTKGTTCGQAIADSGR
MENAYVRQAREHDPVRIGIDKAISETARRLRHILDTHGPQALAFYVSGQMSLEAQYLINK
LAKGFVRTHHIESNSRLCMASAGSGYKLSLGADGPPGSYEDFDRADLFFVIGANMADCHP
ILFLRMMDRVKAGAKLIVVDPRRNATADKANLFLPIKPGTDLALLNGLLHLLVKNGHTDP
AFIAAFTDGWEAMPAFLEDYSPEHVAVITGLAEADIRQAADWIGQAAEWMSCWTMGLNQS
THGTWNTNALCNLHLATGAICRPGSGPFSLTGQPNAMGGREMGYMGPGLPGQRSVLVEAD
RAFIEDLWQIPPDSLPRQAGAGTVGMFEQMAAGQIKACWIICTNPVASVPNRQTVIKGLQ
TAELVITQDAFLDTETNRYADILLPGALWAEAEGVMINSERNLTLMQKAVDAPGETLPDW
QIIARVASEMGFAEAFTYASASEVFEEIKRAWNPKTGYDIRGASYPRLREKPLQWPCASD
TAADRNPIRYVDKGPVTFATDNGKAQFLARPHMPPAELPDEAFPFVLNTGRVQHQWHTLT
KTGKVATLNKLNPGPFVELHAEDAARLGIKDKDRVEIRSRRGHAVLPAVITDRVRPGNCF
APFHWNDVFGDNLAINAVTNDAVDPISLQPEFKCCAVALARVELIGLQSLDRPSPVAEDT
AMSRLDAFAEIAGIRHLSAPPLSDSERTYLAGFLSGLQANAARQAVGIPTMPTNAPLADA
SRLWLNGLLGALFSPTESLATPSPAVTLLWASQTGNAEALAERFAKRLRDAGISVELSAM
SDFPASKLASTHTLALISSTFGDGDPPDNGEGFWHSLSTAETRLESLRFAVLALGDPNYD
QFCNHGKQLDQRLLELGATRLLERVDCDTEFEALADAWLVRFQQTLTPAKPVALATPAGK
TKLYGSRLLLNRQLNPLSAHKETRQFALDLADSGLTYEAGDALGVRPRNCPELVNELLDL
TRLKASTCVNIDTFGDVPLQQALTQHFEIARPSSDTLAFIAERSANPGLKHLLNPEHKAE
LNDWLWGRQLADVLQEYPIECSADELLGTLKRLQPRLYSIASSAKAHPHEVHLTVAAVRY
GKRKGVSSTFLADRVGDGEVPLFVQPSKHFRTPTDGDVPMIMIGPGTGIAPFRAFLQERR
ALGHQGRNWLFFGEQHAASDFYYQDELQGMQRDGLLSHLSLAFSRDQAQKVYVQDRIREQ
GAELWRWLQDGAKLYICGDASHMAKDVDQALRHVAQTHGGLGVEGAVDYWRQLSEQKRYL
RDVY