Protein Info for GFF2868 in Variovorax sp. SCN45
Annotation: Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 50% identical to GYAR_THEON: Glyoxylate reductase (gyaR) from Thermococcus onnurineus (strain NA1)
KEGG orthology group: K00090, gluconate 2-dehydrogenase [EC: 1.1.1.215] (inferred from 95% identity to vpe:Varpa_1947)MetaCyc: 46% identical to glyoxylate reductase (Pyrococcus furiosus DSM 3638)
Glyoxylate reductase. [EC: 1.1.1.26]
Predicted SEED Role
"Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-)" in subsystem Glycolate, glyoxylate interconversions (EC 1.1.1.-, EC 1.1.1.215, EC 1.1.1.26, EC 1.1.1.79, EC 1.1.1.81)
MetaCyc Pathways
- ketogluconate metabolism (6/8 steps found)
- L-serine biosynthesis II (3/4 steps found)
- formaldehyde assimilation I (serine pathway) (9/13 steps found)
- L-arabinose degradation IV (5/8 steps found)
- D-xylose degradation IV (3/7 steps found)
- superpathway of pentose and pentitol degradation (16/42 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of type II polyketide products
- Biosynthesis of unsaturated fatty acids
- Bisphenol A degradation
- Butanoate metabolism
- C21-Steroid hormone metabolism
- Fructose and mannose metabolism
- Glycine, serine and threonine metabolism
- Glyoxylate and dicarboxylate metabolism
- Insect hormone biosynthesis
- Linoleic acid metabolism
- Nucleotide sugars metabolism
- Pentose phosphate pathway
- Polyketide sugar unit biosynthesis
- Pyruvate metabolism
- Retinol metabolism
- Tetrachloroethene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.215, 1.1.1.79, 1.1.1.81
Use Curated BLAST to search for 1.1.1.- or 1.1.1.215 or 1.1.1.26 or 1.1.1.79 or 1.1.1.81
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (328 amino acids)
>GFF2868 Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (Variovorax sp. SCN45) MSKPKILVARAIFPETIERLSQHFEVESNQSDESWSKDQLIAKLQGKAGAFTTGSERIDA AVLDACPDLKICANMAVGYNNFDVDAMAAHGVLGTNAPDVLTETTADFGFALLMATARRI TESEHFLRAGKWQKWSFDMFAGSDIHGSTLGIIGMGRIGQGIAKRGAHGFGMKVIYHNRS KLDASLEAECKASYVGKEELLKTADHVVLVVPYSAASHHTIGAAEIALMKPTATLVNIAR GGIVDDAALAVALREKRIAAAGLDVFEGEPKVHPDLLTVPNVVLTPHIASATVPTRRAMA DLAADNLIAYFGGKGPLTPVTPVPAASK