Protein Info for GFF2867 in Sphingobium sp. HT1-2

Annotation: Protocatechuate 4,5-dioxygenase alpha chain (EC 1.13.11.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02792: protocatechuate 4,5-dioxygenase, alpha subunit
  • amino acids 12 to 128 (117 residues), 196.2 bits, E=8.2e-63
• PF07746: LigA
  • amino acids 32 to 117 (86 residues), 117.2 bits, E=1.4e-38

Best Hits

Swiss-Prot: 70% identical to PCYA_SPHSK: Protocatechuate 4,5-dioxygenase alpha chain (ligA) from Sphingobium sp. (strain NBRC 103272 / SYK-6)

KEGG orthology group: K04100, protocatechuate 4,5-dioxygenase, alpha chain [EC: 1.13.11.8] (inferred from 83% identity to npp:PP1Y_Mpl10445)

MetaCyc: 70% identical to protocatechuate 4,5-dioxygenase alpha chain (Sphingomonas sp. SYK6)
Protocatechuate 4,5-dioxygenase. [EC: 1.13.11.8]; 1.13.11.- [EC: 1.13.11.8]; RXN-10889 [EC: 1.13.11.8]

Predicted SEED Role

"Protocatechuate 4,5-dioxygenase alpha chain (EC 1.13.11.8)" in subsystem Central meta-cleavage pathway of aromatic compound degradation (EC 1.13.11.8)

MetaCyc Pathways

• protocatechuate degradation I (meta-cleavage pathway) (8/8 steps found)
• superpathway of vanillin and vanillate degradation (9/10 steps found)
• methylgallate degradation (6/6 steps found)
• 3,4-dichlorobenzoate degradation (3/4 steps found)
• syringate degradation (8/12 steps found)
• 3-chlorobenzoate degradation II (via protocatechuate) (3/6 steps found)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation
• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 1.13.11.8

Use Curated BLAST to search for 1.13.11.8

Protein Sequence (134 amino acids)

>GFF2867 Protocatechuate 4,5-dioxygenase alpha chain (EC 1.13.11.8) (Sphingobium sp. HT1-2)
MSIDIHEYLAEFDDIPGTRVYTAARARQGYWMNQFAMSLMKAENRVRWLAGERAYIDEWP
MTEAQKQAILDRDYNRCLDLGGNIYFLAKVFSTDGLSFLQAVGTMTGMSPEDYQAMMIAG
GRSPQGVRSIKEKR