Protein Info for GFF2846 in Xanthobacter sp. DMC5

Annotation: HTH-type transcriptional regulator PerR

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF00126: HTH_1" amino acids 6 to 65 (60 residues), 68.4 bits, see alignment E=4.3e-23 PF03466: LysR_substrate" amino acids 92 to 288 (197 residues), 102.6 bits, see alignment E=2.1e-33

Best Hits

Swiss-Prot: 53% identical to Y4MQ_SINFN: Uncharacterized HTH-type transcriptional regulator y4mQ (NGR_a02420) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: None (inferred from 72% identity to xau:Xaut_1542)

Predicted SEED Role

"Glycine cleavage system transcriptional activator" in subsystem Orphan regulatory proteins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (295 amino acids)

>GFF2846 HTH-type transcriptional regulator PerR (Xanthobacter sp. DMC5)
MKRLPLSALQAFESAARRGSFRVAGEELGISPSAISHAVRKLEDLMGAALFEREGRAVRL
NPAGEALMRHVAAGFDEMRQGIEMVGARSVNLLRLHCAPSLAAQWLLPRLRTLLAKQPGL
EVRLAAGVDYPRFEHDEFDADICYGPPRQEGLIVVPLGEETVTPLCAPEMAARISGPLDL
LDHDLIESDNKRVRWNHWFESNGVAPPGPRGSRFDRSFMAIAAAVDGLGIALESTRLAER
EIARGQLVAPLAGKANDVTYVGHYLVFPRAAKARRAVRMFATWITQELDLPTPRM