Protein Info for GFF2841 in Sphingobium sp. HT1-2

Annotation: HlyD-like membrane fusion protein YhiI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 363 transmembrane" amino acids 7 to 28 (22 residues), see Phobius details PF16576: HlyD_D23" amino acids 47 to 300 (254 residues), 38.1 bits, see alignment E=2.1e-13 PF13437: HlyD_3" amino acids 215 to 317 (103 residues), 53.2 bits, see alignment E=9.4e-18

Best Hits

Swiss-Prot: 58% identical to YHII_ECOLI: Uncharacterized protein YhiI (yhiI) from Escherichia coli (strain K12)

KEGG orthology group: K01993, HlyD family secretion protein (inferred from 68% identity to cak:Caul_1949)

Predicted SEED Role

"HlyD family secretion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (363 amino acids)

>GFF2841 HlyD-like membrane fusion protein YhiI (Sphingobium sp. HT1-2)
MSMNKRWIVRGLIVAAVAGIAFLLWTLLKPAGLPEGIVGGNGRLEATEIDVASKSAGRIK
DIVADEGQLVKAGEIVAHMDVDVLDAQRTEAVAQLAEAQNAVLVAQTQVAQRESEKAAAQ
AVVRQRIAELNASRKRLARSETLAREGATAVQERDDDAAQTEGAAAAVEAARAQLASAEV
AITNARNQVIGSRSRVEAGRATIQRIEADINDSSLRAPRDGRVQYRVAQPGEVVTAGGRV
LSLVDLSDVTISFFLPATAAGRVAIGSEARIVLDAIPDYPIPARISFVADVAQFTPKTVE
TQSEREKMMFRVKARIDPALLRKHIAQVKTGLPGMAYVRINPGVAWPASLAVRALPEGSR
AVQ