Protein Info for PS417_14495 in Pseudomonas simiae WCS417

Annotation: maltooligosyl trehalose synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 912 TIGR02401: malto-oligosyltrehalose synthase" amino acids 7 to 904 (898 residues), 953.7 bits, see alignment E=4.9e-291 PF00128: Alpha-amylase" amino acids 28 to 168 (141 residues), 48.3 bits, see alignment E=4.9e-17

Best Hits

KEGG orthology group: K06044, (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase [EC: 5.4.99.15] (inferred from 90% identity to pfs:PFLU3367)

Predicted SEED Role

"Malto-oligosyltrehalose synthase (EC 5.4.99.15)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 5.4.99.15)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UCG2 at UniProt or InterPro

Protein Sequence (912 amino acids)

>PS417_14495 maltooligosyl trehalose synthase (Pseudomonas simiae WCS417)
MKALPLRATQRLQFHKGFTLDDAVPLVPYFARLGISHLYASPLLSARAGSMHGYDVVDPT
RVNPELGGEAALRRLVAALREHGMGLILDIVSNHMAVGGADNPWWLDLLEWGRMSPYSEF
FDIQWHSPDPLLKGQLLMPFLGSDYGEALQNGTVSLQFDATHGAFYVEHYEHRFPICPRD
YATILGNDELLRPLSDRFSALAYQDDAYHEAAWLKQALAERATEEPVRQAIEAQLATFDF
DRLHNLLEQQAYRLASWRTAADDINWRRFFDVNELGGLRVERTAVFEATHGKIFELISEG
LVDGLRIDHIDGLADPRGYCRKLRRRVDSLAPDRHLPIFVEKILGEGETLRTDWQVDGTT
GYEFMNQLSLLQHHPDGFEPLAQLWTRHSERPAAFIEEARLARQQILNGSLGGDFESVAQ
ALLQVARDDVMTRDLTLGAIRRALQELIVHFPVYRTYISARGRSDADDTLFKQAMDGART
TLGEGDWPVLDHLEKWLGGEPWRNRPVGRERKILKHACVRFQQLTSPAAAKAVEDTAFYR
SAVLLSRNDVGFSTEQFSAPLSDFHAVNQQRLQTFPHNLLATATHDHKRGEDTRARLAVL
SECAPWYVEQVEHWRTLAAPLRTDASTPSAGDELILYQVLLGSWPLDQGADFAAYQQRLW
QWQQKALREAKLQSSWSAPNEAYEQGVEAFLSRLLLSEDGRALRTAIGNAAQAIAPAGAL
NGLAQSLLRLTVPGVPDLYQGVEFWDFSLVDPDNRRPVDFSARQEALATPADIGELLFNW
RDGRIKQALIAQVLAVRMAHPELFRSATYTPLEVVGKHAERVVAFCREHQGKRLLVVVPR
WPHDLLENGVHPQINAQVWGDTRVKLPFAATTQNWKGLFHTGAVTPDKELLVSAALGDFP
VNVFINPDDQES